食品科学2017，Vol.38Issue(21)：170-174,5.DOI:10.7506/spkx1002-6630-201721027
市售鸡肉及内脏中磺胺耐药菌污染特征
Prevalence and Characteristics of Sulfamethoxazole Resistant Bacteria in Retail Chicken Meat and Giblets
摘要
Abstract
In order to investigate the potential risk of retail chicken products in transmitting antibiotic resistance (AR) encoding genes,sulfamethoxazole/trimethoprim resistant (Sul/Trir) bacteria were isolated from 13 chicken products,including nine chicken meat samples and four giblet samples,purchased from five supermarkets and local markets.The Sul/Trir resistance genes were mapped by Southern blot analysis.Sul/Trir bacteria were detected in all chicken samples.The prevalence of Sul/Trir bacteria ranged from 2.0 × 102 CFU/g to 1.0 × 107 CFU/g,accounting for 1.42％-82.00％ of the total c.ultivable bacteria.Multi-drug resistance was observed for all 297 Sul/Trir isolates,and the positive rates of AR genes including sull,sul2,tetA,tetB,tetC,tetE,tetG,tetL,tetM and intI were 11.33％,32.81％,6.64％,12.89％,2.73％,6.25％,9.38％,18.75％,39.84％ and 28.52％ respectively.Escherichia sp.,Pedobacter sp.,Staphylococcus sp.,Enterococcus sp.,Sphingobacterium sp.and Acinetobacter sp.were identified as hosts for AR genes.The minimum inhibitory concentration (MIC) of Sul for most isolates was higher than 512 μtg/mL.Furthermore,a plasmid harboring sul2 gene was found in one Sul/Trir isolate by Southern blot hybridization.In conclusion,retail chicken products can be a potential avenue transmitting antibiotic resistant bacteria and AR genes.关键词
磺胺耐药菌/耐药基因/鸡肉/内脏Key words
sulfamethoxazole/trimethoprim resistant bacteria/antibiotic resistance genes/chicken meat/giblets分类
轻工纺织引用本文复制引用
李姝,邵毅,周昌艳,王华,邢增涛,赵志辉..市售鸡肉及内脏中磺胺耐药菌污染特征[J].食品科学,2017,38(21):170-174,5.基金项目
国家自然科学基金青年科学基金项目(31401599) （31401599）
上海市科技兴农重点攻关项目(应用基础类)(沪农科攻字(2014)第7-3-6号) （应用基础类）
上海市青年科技英才扬帆计划项目(14YF1413000) （14YF1413000）
美国外交部国际教育协会美英联合全球创新倡议(GⅡ)项目(GRT00033913) （GⅡ）
