食品科学2017，Vol.38Issue(21)：217-222,6.DOI:10.7506/spkx1002-6630-201721035
亚硒酸钠与白藜芦醇联合应用对肺癌细胞的协同效应分析
Synergistic Effect of Sodium Selenite and Resveratrol on Lung Cancer Cell Line
摘要
Abstract
Objective:To investigate the effect of sodium selenite,resveratrol and their combination on the proliferation and apoptosis of lung cancer cell line A549 cells and to explore the underlying mechanisms.Methods:Methyl thiazolyl tetrazolium (MTT) method was used to assess the individual and combined effects of sodium selenite and resveratrol on proliferation and survival ofA549 cells.The cell apoptosis rate and cell cycle were determined by flow cytometry.Results indicated that sodium selenite and resveratrol had an antagonistic effect on A549 cells in a concentration-dependent manner,and the effect was very significant at a concentration ≥ 5 μmol/L for sodium selenite and ≥ 65 μmol/L for resveratrol (P ＜ 0.01).The median lethal doses of (LD50) of sodium selenite and resveratrol for A549 cells were 4.759 and 45.24 μmol/L,respectively.However,when sodium selenite and resveratrol were used in combination,a synergistic effect was observed.The combination of low-dose sodium selenite and resveratrol inhibited A549 cells as effectively as high-dose sodium selenite,greatly reducing the cytotoxicity of high-dose sodium selenite.In addition,the combination of sodium selenite and resveratrol arrested the cell cycle at the G0/G1 phase and ultimately led to cell apoptosis.关键词
亚硒酸钠/白藜芦醇/肺癌细胞A549/细胞周期/细胞凋亡Key words
sodium selenite/resveratrol/lung cancer A549 cells/cell cycle/cell apoptosis分类
轻工纺织引用本文复制引用
张平,万鹰昕,李楠..亚硒酸钠与白藜芦醇联合应用对肺癌细胞的协同效应分析[J].食品科学,2017,38(21):217-222,6.基金项目
北京市教委面上项目(KM201711417008) （KM201711417008）
北京联合大学“启明星”大学生科技创新项目 （）
