食品科学2017，Vol.38Issue(21)：223-229,7.DOI:10.7506/spkx1002-6630-201721036
鲟鱼硫酸软骨素对结直肠癌细胞抑制作用
Inhibitory Effect of Chondroitin Sulfate from Sturgeon Bone on Colorectal Cancer Cells
摘要
Abstract
Objective:This study is aimed to investigate the inhibitory effect of chondroitin sulfate from sturgeon bone on the proliferation of colorectal cancer cells and to elucidate the underlying mechanism.Methods:Three representative colorectal cancer cells (Caco-2,HCT-116,and SW480) were used in this study.Cell growth and proliferation was tested by cell counting kit-8 (CCK-8) method.Colorectal cancer cell line HCT-116 was used to further investigate the pro-apoptotic ability of sturgeon chondroitin sulfate.Cell cycle and apoptosis were determined by flow cytometry.Cell morphology was observed after cell staining.Apoptosis was evaluated by caspase-3 activity.Our study indicated that sturgeon chondroitin sulfate significantly inhibit the cell proliferation of Caco-2,HCT-116 and SW480 cells with a percentage suppression of 70.94％,90.00％ and 75.00％,respectively.After sturgeon chondroitin sulfate treatment,the G1/G0 phase increased to 88.56％ and the S phase declined to 4.47％.Meanwhile,sturgeon chondroitin sulfate-treated cells exhibited morphological changes,nuclear pyknosis and cell fragmentation,and significantly increased apoptotic enzyme activity (1 645 IU/μg) with an apoptosis rate of 63.73％.Sturgeon chondroitin sulfate showed potential anticancer activity,which will make it a new functional food ingredient.关键词
鲟鱼硫酸软骨素/结直肠癌/细胞增殖/凋亡Key words
sturgeon chondroitin sulfate/colorectal cancer/cell proliferation/apoptosis分类
生物科学引用本文复制引用
武瑞赟,刘蕾,张金兰,陈贵林,李平兰..鲟鱼硫酸软骨素对结直肠癌细胞抑制作用[J].食品科学,2017,38(21):223-229,7.基金项目
北京市鲟鱼、鲑鳟鱼创新团队项目(SCGWZJ201711) （SCGWZJ201711）
