食品科学2017，Vol.38Issue(21)：230-240,11.DOI:10.7506/spkx1002-6630-201721037
干酪乳杆菌Qian增强京尼平苷对盐酸/乙醇诱导小鼠胃损伤的预防效果
Lactobacillus casei Qian Enhances the Preventive Effect of Geniposide on HCl/Ethanol Induced Gastric Injury in Mice
摘要
Abstract
The aim of this study was to investigate the synergistic effect of a recently discovered strain Lactobacillus casei Qian (LC-Qian) and geniposide on the prevention of ethanol-induced gastric injury in mice.LC-Qian (0.5 × 10s CFU/kg mb)was mixed with geniposide (50 mg/kg mb),and LC-Qian (0.5 × 108 CFU/kg mb) and geniposide (50 mg/kg mb) were also used separately to protect against HC1/ethanol induced gastric injury and to compare their preventive effects.LC-Qian combined with geniposide (the combination group) diminished the gastric lesion area and gastric secretion volume and raised the pH of the gastric juice to a level close of that of the control group and significantly different from that in the model group.The combination treatment was also able to decrease the serum levels of cytokines such as interleukin-6 (IL-6),IL-12,tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ),motilin (MOT),substance P (SP),and endothelin-1 (ET-1) but increase the levels of somatostatin (SS) and vasoactive intestinal peptide (VIP) as compared to the model group.The combination group showed higher NO contents in serum,heart,liver,kidney and stomach in comparison to the model group,but their levels were lower than those in the normal group.As indicated by reverse transcription polymerase chain reaction (RT-PCR) assay,the combination treatment could increase the mRNA expression levels of neuronal nitric oxide synthase (nNOS),endothelial NOS (eNOS) and inhibitor kappa B-α (IκB-α),and decrease the mRNA expression levels of inducible nitric oxide synthase NOS (iNOS) and nuclear factor kappa B (NF-κB) as compared to the model group.These expression levels in the combination group were similar to those of the normal group.From these results,it can be concluded that the combination treatment has the best preventive effects against gastric injury and LC-Qian could enhance the preventive effect of geniposide against gastric injury.Therefore,this combination treatment could be used as a new way method for the prevention of gastric injury.关键词
干酪乳杆菌Qian/乙醇/mRNA表达/小鼠/胃Key words
Lactobacillus casei Qian/ethanol/mRNA expression/mouse/stomach分类
轻工纺织引用本文复制引用
骞宇,索化夷,易若琨,李贵节,赵欣..干酪乳杆菌Qian增强京尼平苷对盐酸/乙醇诱导小鼠胃损伤的预防效果[J].食品科学,2017,38(21):230-240,11.基金项目
重庆高校创新团队建设计划项目(CXTDX201601040) （CXTDX201601040）
重庆市工程技术研究中心建设项目(cstc2015yfpt_gcjsyjzx0027) （cstc2015yfpt_gcjsyjzx0027）
重庆市基础与前沿研究计划一般项目(cstc2016jcyjA0339) （cstc2016jcyjA0339）
重庆第二师范学院校级科研项目(KY2015TBZC) （KY2015TBZC）
