中国食物与营养2017，Vol.23Issue(9)：28-30,3.
HPLC-DAD和LC/MS/MS测定豆奶中三聚氰胺
Determination on Melamine in Soy Milk by HPLC-DAD and LC/MS/MS
尤妍1
作者信息
- 1. 玉环市食品药品检验检测中心,浙江台州317608
- 折叠
摘要
Abstract
[Objective] To establish a method for the melamine in soy milk by HPLC-DAD and LC/MS/MS.[Method] we used trichloroacetic acid and acetonitrile to extract and precipitate impurities.The Extraction was purificated by purification column.[Result] Using LC/MS/MS method,the detection limit was melamine was 0.001 5 mg/kg,the calibration curves showed a good linearity in the range of 0.01 ～ 0.5 μg/mL,and the correlation coefficients (R2) was 0.999 8.The recoveries ranged from 85％ ～ 89％.The method was applicable to detect low concentration of sample.While using HPLC-DAD method,the detection limit of melamine was 0.024 mg/kg,the calibration curves showed a good linearity in the range of 0.5 ～ 100 μg/mL,and the correlation coefficients (R2) was 0.999 9.The recoveries ranged from 83％ ～ 91％.It was suitable for high concentration of the detection of the sample.[Conclusion] The two kinds of detection methods used in combination could detect 0.01 ～ 100 mg/kg of melamine,greatly expand the detection range.关键词
二极管阵列高效液相色谱/三重四级杆液质联用/三聚氰胺/豆奶Key words
Diode Array High Performance Liquid Chromatography (HPLC-DAD)/Triple Level 4 Pole Liquid Ms (LC/MS/MS)/melamine/soy milk引用本文复制引用
尤妍..HPLC-DAD和LC/MS/MS测定豆奶中三聚氰胺[J].中国食物与营养,2017,23(9):28-30,3.
