中国食物与营养2017，Vol.23Issue(9)：36-40,5.
青海蚕豆中原花青素和左旋多巴的含量测定和品种间差异的比较
Determination of Oligomeric Proanthocyanidins and Levodopa in Broad Bean from Qinghai Province and Comparison of Differences Among Varieties
摘要
Abstract
[Objective] To establish a method for determination of levodopa and oligomeric proanthocyanidins (OPC) content for measuring 22 batches of samples.[Method] Detection wavelength of UV spectrophotometric method for OPC was 554nm.HPLC method was established to detect the content of L-dopa in broad bean:column:hanbon ODS-2 (250mm * 4.6mm,5 μm),nobile phase:methanol-0.1 moL/L acetic acid solution (5:95);flow rate 0.8 mL/min;detection wavelength 280 nm;column temperature 25℃;sample size 20μL [Result] The results showed that the range of 75 ～375μg/mL was good linear for OPC and L-DOPA.[Conclusion] The two methods were stable and reliable.The content of the 20 batches of samples were determined,and the changes of the content before and after processing were compared.关键词
青海蚕豆/左旋多巴/原花青素/紫外分光光度法/HPLCKey words
broad hean/levodopa (L-DOPA)/oligomeric proanthocyanidin (OPC)/Ultraviolet Spectrophotometry/HPLC引用本文复制引用
申士富,钱静,刘廷,李俊松,赵永贵,狄留庆..青海蚕豆中原花青素和左旋多巴的含量测定和品种间差异的比较[J].中国食物与营养,2017,23(9):36-40,5.基金项目
江苏省重点研发专项资金项目(项目编号:BF2016004) （项目编号:BF2016004）
青海省星火计划项目(项目编号:2014-NS-306) （项目编号:2014-NS-306）
江苏高校优势学科建设工程资助项目(项目编号:PAPD) （项目编号:PAPD）
江苏省“青蓝工程”创新团队支持计划资助(项目编号:QLP). （项目编号:QLP）
