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动物双歧杆菌RH对消化道的耐受性及其微胶囊制备的研究

李军 张丽末 桂萌 刘蕾 郭秀锋 李平兰

食品科学2017,Vol.38Issue(22):14-21,8.
食品科学2017,Vol.38Issue(22):14-21,8.DOI:10.7506/spkx1002-6630-201722003

动物双歧杆菌RH对消化道的耐受性及其微胶囊制备的研究

Resistance of Bifidobacterium RH to Simulated Gastrointestinal Conditions and Preparation of Microcapsules Containing This Strain

李军 1张丽末 1桂萌 2刘蕾 1郭秀锋 1李平兰1

作者信息

  • 1. 北京食品营养与人类高精尖创新中心,中国农业大学食品科学与营养工程学院,北京 100083
  • 2. 北京市水产科学研究所,北京 100071
  • 折叠

摘要

Abstract

The aim of this study was to evaluate the potential of Bifidobacterium RH to be used as a probiotic product.We examined the viability of the probiotic under simulated gastrointestinal conditions and we investigated the production of probiotic microcapsules with resistance to various negative factors.The bacterial counts were 107 CFU/mL and 106 CFU/mL,respectively,under simulated gastrointestinal conditions separately and sequentially.The optimized wall material of microcasuples contained 10% glycerol,0.50% sodium alginate,1.50% whey protein,1.25% Arabic gum and 1.25% soybean lecithin as determined by one-factor-at-a-time and orthogonal array designs.The count of viable Bifidobacterial cells in the microcapsules was 1.80 × 1012 CFU/g,and with respect to encapsulation efficiency,the results showed a mean value of 96.04%.Thus,the study demonstrated that microencapsulated Bifidobacterium RH could be resistant to simulated gastrointestinal conditions.The microencapsulation process was shown to efficiently increase the viability of probiotic cultures,and the microorganism was released from the microcapsules.This study may provide a theoretical and technical support for the development of probiotic products containing Bifidobacterium RH.

关键词

动物双歧杆菌RH/消化道逆环境/冻干微胶囊

Key words

Bifidobacterium RH/simulated gastrointestinal conditions/microcapsules

分类

生物科学

引用本文复制引用

李军,张丽末,桂萌,刘蕾,郭秀锋,李平兰..动物双歧杆菌RH对消化道的耐受性及其微胶囊制备的研究[J].食品科学,2017,38(22):14-21,8.

基金项目

国家自然科学基金面上项目(31271827 ()

31671831) ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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