食品科学2017，Vol.38Issue(22)：41-47,7.DOI:10.7506/spkx1002-6630-201722007
基于AI-2介导的类植物乳杆菌生物被膜态与浮游态抗胁迫能力比较与分析
Stress Resistance of Biofilm and Planktonic Cells of Lactobacillus paraplantarum L-ZS9 Regulated by Autoinducer 2
摘要
Abstract
The present study compared the heat,acid and bile resistance of biofilm and planktonic cells of L-ZS9 by colony counting method.The results showed that biofilm cells had stronger stress resistance than their planktonic counterpart.The transcriptional levels of the stress resistance-related genes atpβ,atpε,clp,pspC and ccpA and the luxS gene encoding the quorum sensing signal autoinducer 2 (AI-2) synthase were investigated in biofilm and planktonic cells through real-time PCR.The results showed that the transcriptional levels of these genes were up-regulated in biofilm cells compared to planktonic cells.AI-2 activity were measured by its ability to induce luminescence in the reporter strain Vibrio harveyi BB 170 and the results showed that the supematant of biofilm cells had higher AI-2 activity than planktonic cells.Exogenous AI-2 could regulate the transcriptional levels of the atpβ,atpε,clp,ccpA and luxS genes.This study suggested that biofilm protected the cells by physical defense and biofilm ceils differed from planktonic cells in terms of the transcriptional levels of the investigated genes.The quorum sensing system played an important role in the formation and regulation of biofilm.This work has laid a foundation for producing functional foods based on biofilm probiotics with high activity.关键词
类植物乳杆菌/生物被膜/抗胁迫能力/信号分子AI-2/转录水平Key words
Lactobacillus paraplantarum L-ZS9/biofilm/stress resistance/signal AI-2/transcription分类
生物科学引用本文复制引用
刘蕾,武瑞赟,李军,李平兰..基于AI-2介导的类植物乳杆菌生物被膜态与浮游态抗胁迫能力比较与分析[J].食品科学,2017,38(22):41-47,7.基金项目
国家自然科学基金面上项目(31671831 （）
31471707) （）
北京市鲟鱼、鲑鳟鱼创新团队项目(SCGWZJ201711) （SCGWZJ201711）
