食品科学2017，Vol.38Issue(22)：231-238,8.DOI:10.7506/spkx1002-6630-201722035
响应面试验优化红花籽油水酶法提取工艺
Optimization of Aqueous Enzymatic Extraction of Safflower Oil by Response Surface Methodology
摘要
Abstract
The aqueous enzymatic extraction of safflower oil was optimized using factional factorial design and response surface methodology.The effects of processing parameters,including enzyme type and combinations,material to liquid ratio,total enzyme dosage,hydrolysis time,temperature and pH on oil yield were investigated.Results showed that a mixture of xylanase (UTC-X50),pectinase (NCB3/ZG-040) and alkaline protease (NCB3/ZG-002) (1∶2∶3,U/U) was the most efficient for the extraction of safflower oil and the optimum enzyme dosage was 197.36 U/g.The maximum yield of 84.68％ was obtained after sequential hydrolysis with both xylanase and pectinase for 131 min at pH 4.2 and 50 ℃ with a material/liquid ratio 1∶4,followed by alkaline protease at pH 9.8 and 40 ℃ for 60 min.Gas chromatography analysis revealed that the fatty acid profile of safflower oil obtained under the optimized conditions contained 78.27％ linoleic acid,12.61％ oleic acid and 0.10％ linolenic acid.关键词
红花籽油/水酶法/响应面/脂肪酸Key words
safflower oil/aqueous enzymatic method/response surface methodology/fatty acid分类
轻工纺织引用本文复制引用
李晓,李春阳,曾晓雄,王帆..响应面试验优化红花籽油水酶法提取工艺[J].食品科学,2017,38(22):231-238,8.基金项目
江苏省粮油品质控制及深加工重点实验室开放课题(LYPK201502) （LYPK201502）
