食品科学2017，Vol.38Issue(22)：309-316,8.DOI:10.7506/spkx1002-6630-201722046
石斑鱼中阿维菌素类药物多残留测定及食用安全风险评估
Determination and Food Safety Risk Assessment of Avermectin Residues in Grouper
摘要
Abstract
A multi-residue method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the quantitative determination of abamectin,ivermectin and emamectin benzoate in grouper plasma,muscle and liver.The target analytes were extracted with acetonitrile and then cleaned up with an alkaline alumina column/LC-C18 SPE column.The analytes were separated on a Thermo Hypersil Gold C18 column by gradient elution with 0.1％ formic acid-10 mmol/L ammonium acetate as mobile phase A and acetonitrile as mobile phase B,and detected by multiple reaction monitoring (MRM) with electrospray ionization (ESI) under positive ion mode.The target compounds were quantified by the matrix-matched external standard method.Both pesticides could move into water through various environmental routes.Therefore,the bioaccumulation and elimination of avermectin and ivermectin in groupers were studied by bath administration at the upper and lower concentration limits (4 and 8 ng/mL for avermectin,and 6 and 12 ng/mL for ivermectin) in environmental water.Meanwhile,the food safety risk of the pesticide residues in fish was assessed.The results showed that the calibration curves were linear (R2＞ 0.99) in the concentration range of 2.5-200 ng/mL for abamectin and ivermectin and 0.25-20 ng/mL for emamectin benzoate.The limits of detection (LOD) for abamectin,ivermectin and emamectin benzoate were 2.5,2.5 and 0.25 ng/mL in plasma,1,1 and 0.1 μg/kg in muscle,2.5,2.5 and 0.25 μg/kg in liver,respectively.The limits of quantification (LOQ) were 5,5 and 0.5 ng/mL in plasma,2,2 and 0.2 μg/kg in muscle,5,5 and 0.5 μg/kg in liver,respectively.The average recoveries at three spiked levels ranged from 74.6％ to 93.6％.Intra-day and inter-day relative standard deviations (RSDs) were 2.3％-10.9％ and 9.2％-12.6％,respectively.Abamectin and ivermectin were no-bioaccumulative substances and their elimination processes in grouper conformed to a first order kinetics equation.Under the conditions of this study,drug concentration was an important factor affecting the residual drug concentration and elimination time in grouper muscle tissues.Gouper was safe for consumption 22 and 39 days after 72 h bath administration for 4-8 ng/mL abamectin and 6-12 ng/mL ivermectin,respectively.关键词
石斑鱼/阿维菌素类药物残留/食用安全风险评估Key words
grouper/avermectin residues/food safety risk assessment分类
医药卫生引用本文复制引用
钱卓真,汤水粉,罗方方,王丽娟,位绍红..石斑鱼中阿维菌素类药物多残留测定及食用安全风险评估[J].食品科学,2017,38(22):309-316,8.基金项目
福建省海洋经济创新发展区域示范项目(闽台重要海洋生物资源高值化开发技术公共服务平台,2014FJPT01) （闽台重要海洋生物资源高值化开发技术公共服务平台,2014FJPT01）
厦门南方海洋研究中心项目(福建重要海洋经济生物种质库与资源高效开发技术公共服务平台,14PZY017NF17) （福建重要海洋经济生物种质库与资源高效开发技术公共服务平台,14PZY017NF17）
福建省海洋与渔业科技项目(KJXH-2010-007) （KJXH-2010-007）
福建省海洋高新产业发展专项(闽海洋高新[2014]18号) （闽海洋高新[2014]18号）
国家海洋局海洋公益性行业科研专项(201505034-4) （201505034-4）
