中国食品学报2017，Vol.17Issue(9)：20-26,7.DOI:10.16429/j.1009-7848.2017.09.003
海参蛋白肽对H2O2诱导RAW264.7细胞氧化损伤的保护作用
Protective Effects of Sea Cucumber Peptides on H2O2-Induced Oxidative Injury in RAW 264.7 Macrophages
摘要
Abstract
The antioxidant activities of sea cucumber peptides,and the protective effects and underlying mechanisms of sea cucumber peptides on H2O2-induced oxidative injury in RAW 264.7 macrophages were investigated in current study.Hydroxyl radical-scavenging capacity,ferrous ion (Fe2+)-chelating ability and ferric reducing activity were determined to assess the antioxidant activities of sea cucumber peptides in vitro.The model of oxidative injury in RAW 264.7 macrophages was established by hydrogen peroxide treatment.The levels of intracellular reactive oxygen species (ROS) and cell viability were assayed using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),respectively.The mRNA expression levels of heme oxygenase-1 (HO-1) were evaluated by real time quantitative polymerase chain reaction.Results showed that sea cucumber peptides possessed hydroxyl radical-scavenging capacity,Fe2+-chelating ability and ferric reducing activity in a dose-dependent manner.Sea cucumber peptides significantly reducedthe levels of intracellular ROS and increased cell viabilityin RAW 264.7 macrophages under oxidative injury (P＜0.05).Moreover,the mRNA expression levels of HO-1 were significantly increased by sea cucumber peptides in RAW 264.7 macrophages (P＜0.05).The improvement effects of sea cucumber peptides on the cell viability of RAW 264.7 macrophages subjected to H2O2-induced oxidative injury were partially reversed by cotreatment with zinc(Ⅱ)-protoporphyrin Ⅸ (ZnPPⅨ),a specific inhibitor of HO-1.These results suggested that sea cucumber peptides protected against H2O2-induced oxidative injury in RAW 264.7 macrophages by increasing HO-1 mRNA expression.关键词
海参蛋白肽/氧化损伤/RAW264.7巨噬细胞/血红素氧合酶-1Key words
sea cucumber peptides/oxidative injury/RAW 264.7 macrophages/heme oxygenase-1引用本文复制引用
宋佳佳,成雪,纪小敏,姜乃义,毛学英..海参蛋白肽对H2O2诱导RAW264.7细胞氧化损伤的保护作用[J].中国食品学报,2017,17(9):20-26,7.基金项目
国家自然科学基金项目(31371753) （31371753）
