中国食品学报2017，Vol.17Issue(9)：236-242,7.DOI:10.16429/j.1009-7848.2017.09.030
产中性耐热β-葡萄糖苷酶芽孢杆菌的筛选与鉴定
Screening and Identification of a Neutral and Heat-tolerant β-Glucosidase-producing Bacillus Strain
摘要
Abstract
Objective:To screen and indentify a neutral and heat-tolerant β-glucosidase-producing Bacillus strain from the soil.Methods:The soil samples were treated and streaked onto the agar-plate containing esculin.After the samples were cultured at 37 ℃ for 48 h,the colonies that can produce larger dark brown hydrolysis cycle were picked and isolated.The strain with the highest β-glucosidase activity was re-screened with pNPG as the substrate,and was identified by the colony morphology in combination with the physiological and biochemical characteristics and the molecular biology technology.Results:20 strains that could produce β-glucosidase were obtained from soil samples.The strain with the highest β-glucosidase activity was obtained by re-screening,and was named ZJ1308 and identified as Bacillus cereus.Its β-glucosidase activity was up to 0.46 U/mL.Conclusion:This study lays a good foundation for the industrial application of β-glucosidase.关键词
β-葡萄糖苷酶/芽孢杆菌/生理生化鉴定/16S RdnaKey words
β-glucosidase/Bacillus/physiological and biochemical identification/16S rDNA引用本文复制引用
于平,李鹏,陈幸鸽,徐真妮..产中性耐热β-葡萄糖苷酶芽孢杆菌的筛选与鉴定[J].中国食品学报,2017,17(9):236-242,7.基金项目
食品科学与工程浙江省重中之重一级学科项目(20175IAR214) （20175IAR214）
