现代食品科技2017，Vol.33Issue(11)：1-6,6.DOI:10.13982/j.mfst.1673-9078.2017.11.001
双酚S影响3T3-L1前脂肪细胞分化及其作用机制的研究
Effect of BPS on the Differentiation of 3T3-L1 Preadipocytes and Its Mechanism
摘要
Abstract
Bisphenol A (BPA) is restricted for its endocrine disruption and adverse health effects on the human body,so that bisphenol S (BPS) is widely used as a substitute n a variety of food packaging materials.The security of BPS has not been fully studied before its large-scale application..In this study,3T3-L1 preadipecytes were used as an in vitro model to investigate the effect of BPS on the differentiation of 3T3-L1 preadipocytes and its mechanism,to evaluate the safety of BPS and provide reference for the prevention of chronic metabolic diseases such as obesity.The results showed that BPS could promote the growth of 3T3-L1 preadipocytes and could significantly inhibit the proliferation of cells when the concentration was over 400 μmol/L.BPS could significantly induce the accumulation of lipid in the differentiated 3T3-L1 cells which showed a non-dose-dependent relationship.Compared with the model group,BPA up-regulated the expressions of PPARγ,C/EBR and aP2 genes,whereas BPS only seems to up-regulate the expression of PPARγ.In addition,BPS and BPA could significantly decrease the expression of GLUT4 gene (p＜0.01).In conclusion,the pathways of BPS and BPA acting on adipocytes differentiaton were not exactly the same,which might be accomplished under the combined action of various transcription factors.关键词
双酚A/双酚S/3T3-L1细胞/肥胖/内分泌干扰物/食品包装/安全评价Key words
BPS/BPA/3T3-L1 cells/obesity/endocrine disruptor, food packaging/safety evaluation引用本文复制引用
周兴华,张晓伟,孙鑫娟,肖香,赵延胜,祝莹,董英..双酚S影响3T3-L1前脂肪细胞分化及其作用机制的研究[J].现代食品科技,2017,33(11):1-6,6.基金项目
国家自然科学基金资助项目(31501569) （31501569）
江苏大学高级专业人才专项(13JDG037) （13JDG037）
