现代食品科技2017，Vol.33Issue(11)：37-41,5.DOI:10.13982/j.mfst.1673-9078.2017.11.006
ARTP诱变以及基因组重排筛选具有耐高温性能的酿酒酵母
Screening the Saccharomyces Cerevisiae with High Temperature Toleranceby ARTP Mutagenesis and Genome Shuffling
摘要
Abstract
Saccharomyces cerevisiae has the advantages of fast fermentation speed,short fermentation cycle,low viscosity of fermentation raw materials and low production cost during the high temperature fermentation in industrial production,which is more suitable for simultaneous saccharification fermentation technique.Therefore,S.cerevisiae strain with high temperature tolerance is of great significance in the industrial application.In this study,AY12 was used as the starting strain,which was isolated from industrial strains.The strain was mutated by atmospheric and screened at 37 ℃.Then,150 strains with good colony growth were obtained.By the way,the stains were carried out to achieve genome shuffling by sporulation and hybridization,and 137 strains with good growth performance were obtained by re-screening at 37℃.After fermentation in the corn hydrolysate at 35 ℃,14 strains were obtained with good fermentation performance.The strains were rescreened by the simultaneous saccharification fermentation at 35 ℃,and the ethanol yield and residual sugar content of each strain were determined.Finally,7 strains were screened with good tolerance to high temperature fermentation.L-38 was screened as the best fermentation performance strain after the simultaneous sacchadfication fermentation.Compared with the starting strain AY12,the ethanol yield of L-38 was 16.20％ (V/V),which increased by 8.00％.,and the residual sugar content was 35.50 g/L,which was 32.38％ lower than that by AY12.关键词
酿酒酵母/ARTP/基因组重排/耐高温Key words
Saccharomyces cerevisiae/ARTP/genome shuffling/high temperature tolerance引用本文复制引用
赵宇,刘珊珊,陈叶福,郝爱丽,洪坤强,付肖蒙,王鹏飞,肖冬光..ARTP诱变以及基因组重排筛选具有耐高温性能的酿酒酵母[J].现代食品科技,2017,33(11):37-41,5.基金项目
国家重点研发项目(2016YFD0400505) （2016YFD0400505）
“十二五”农村领域国家科技计划(2013AA102108) （2013AA102108）
