现代食品科技2017，Vol.33Issue(11)：134-139,6.DOI:10.13982/j.mfst.1673-9078.2017.11.020
恩诺沙星分子印迹聚合物膜的制备及吸附性能研究
Preparation and Adsorption Properties of Enrofloxacin MolecularImprinted Polymer Film
吕春晖 1方国臻 2王硕2
作者信息
- 1. 天津现代职业技术学院生物工程学院,天津300350
- 2. 天津科技大学食品工程与生物技术学院,天津300222
- 折叠
摘要
Abstract
The enrofloxacin molecular imprinted polymer film was directly synthesized on the surface of polystyrene microtiter plate by surface imprinting technique using the enrofloxacin (ENRO) as template,methacrylic acid (MAA) as functional monomer and ethyleneglycol dimethacrylate (EDMA) as cross-linker.The enrofloxacin molecular imprinted polymer film was characterizedby FT-IR,scanning electron microscope,adsorption-equilibrium experiments,scatchard analysis and adsorption kinetic experiments.The results showed that the imprinted film exhibited good imprinting effect,high specific adsorption ability and fast mass mnsfer rate.Scatchard analysis indicated that the imprinted film contained two types of binding sites with specific binding properties.The dissociation constant (Kd) and maximum binding capacity (Qmax) of the two linear curves were 19.49μg/mL,12.98 μg/mL for the higher affinity sites and 277.78 μg/mL,98.14 μg/mL for the lower affinity sites,respectively,and the heterogeneous of MIP could be ignored in MIAs.The enrofloxacin-specific polymer film synthesized by the proposed method could be used as a biomimetic antibody to determine the enrofloxacin residues in food by competitive immunosorbent assay.关键词
恩诺沙星/分子印迹聚合物膜/表面印迹法/吸附性能Key words
enrofloxacin/molecular imprinted polymer film/surface imprinting/absorption ability引用本文复制引用
吕春晖,方国臻,王硕..恩诺沙星分子印迹聚合物膜的制备及吸附性能研究[J].现代食品科技,2017,33(11):134-139,6.
