现代食品科技2017，Vol.33Issue(11)：257-263,7.DOI:10.13982/j.mfst.1673-9078.2017.11.037
毛细管电泳电化学发光法检测银杏叶中的芦丁
Determination of Rutin in Ginkgo leaf by Capillary Electrophoresis-Electrochemiluminescence
摘要
Abstract
A rapid and efficient separation method was established for the determination of rutin by capillary electrophoresiselectrochemiluminescence (CE-ECL).Tertiary amine compounds,obtained by derivatization reaction of rutin and five secondary amines,were screened by UV,and then detected by CE-ECL.Ginkgo leaf samples were extracted with 70％ ethanol aqueous solution by ultrasonic-assisted extraction.The influences of detection potential,concentration and pH of separation buffer and running buffer,separation voltage,injection voltage and injection time on the separation and detection of rutin were investigated.Under the optimal conditions,rutin was separated in 5 minutes,and the linear concentration range for rutin was 0.002～2 μg/mL.Besides,the regression equation was y=1958.4x+7600.4,with a correlation coefficient of 0.994 and a detection limit of 0.0004 μg/mL (S/N=3).The relative standard deviations (RSD) of migration time,peak height and peak area for seven continuous injections of 0.2 μg/mL rutin were 1.1％,1.2％ and 2.0％,respectively.The proposed method was applied to the determination of the rutin in Ginkgo leaf with the recoveries of 92％-103％.关键词
毛细管电泳电化学发光(CE-ECL)/银杏叶芦丁/衍生法/叔胺类化合物Key words
capillary electrophoresis-electrochemiluminescence (CE-ECL)/rutin in Ginkgo leaf/derivative/tertiary amine compounds引用本文复制引用
魏若男,陈志远,耿敬章..毛细管电泳电化学发光法检测银杏叶中的芦丁[J].现代食品科技,2017,33(11):257-263,7.基金项目
陕西省科技厅农业科技创新与攻关项目(2015NY130) （2015NY130）
陕西理工大学研究生创新基金项目(SLGYCX1623) （SLGYCX1623）
陕西理工大学博士后专项项目(SLGBH16-01) （SLGBH16-01）
