现代食品科技2017，Vol.33Issue(11)：279-284,278,7.DOI:10.13982/j.mfst.1673-9078.2017.11.040
液态奶中硫氰酸钠快速检测试剂盒的研制与应用
Development and Application of Rapid Determination Kit for Sodium Thiocyanate in Liquid Milk
摘要
Abstract
Sodium thiocyanate (NaSCN),a chemical raw material with preservative effect,was often used in dairy products to extend the shelf life,which increased the safety risk.The rapid detection methods for sodium thiocyanate that had been reported had several drawbacks such as serious background interference and low sensitivity.A NaSCN semi-quantitative rapid detection method was established in this study with the principle of sanguineous complex reaction induced by SCNand Fe3+by selecting the chromogenic agent,optimizing chromogenic reaction conditions,simulating milk matrix,pretreating samples and the chromogenic reaction.The rapid determination kit for sodium thiocyanate residues in liquid milk samples was developed.The optimum conditions were as follows:the chromogenic agent,2 mol/L iron nitrate (2 mol/L nitric acid as the solvent);reaction time,2 min.The reaction was complete in room temperature under the optimum conditions.The detection limit of the kit for sodium thiocyanate residues in liquid milk was 5 mg/kg,and the color gradient was well distinguished in the NaSCN concentration of 5～100 mg/kg.The proposed kit was accurate,sensitive,rapid (5 min),stable and not in need of instrument assistance,and wad suitable for on-site screening a large quantities of liquid milk samples for small and medium-sized enterprises and basic units.关键词
硫氰酸钠/快速检测/灵敏度/试剂盒Key words
sodium thiocyanate/rapid determination/sensitivity/kit引用本文复制引用
刘凤银,王淑娟,李轩,李家威,王紫薇,曾思敏,梁岳,穆洪涛..液态奶中硫氰酸钠快速检测试剂盒的研制与应用[J].现代食品科技,2017,33(11):279-284,278,7.基金项目
国家自然科学基金项目(31601540) （31601540）
广东省自然科学基金项目(2016A030310304) （2016A030310304）
大学生创新创业训练计划项目(201614278081) （201614278081）
