茶叶科学2017，Vol.37Issue(6)：551-564,14.
茶树miR156a靶基因SPL6和SPL9的克隆及表达分析
Cloning and Expression Analysis of miR156a-targeted Genes SPL6 and SPL9 in Camellia sinensis
摘要
Abstract
MicroRNA156a-SQUAMOSA promoter binding-like protein(miR156a-SPLs)play important roles in growth process, formation of leaves and response to stress in tea plant (Camellia sinensis). Based on transcriptome data, two cDNA CsSPL6 (2318 bp) and CsSPL9 (1954 bp) were cloned from tea plant. The nucleotide and amino acid sequences, biological information, phylogenetic tree and molecular expression models of the CsSPL6 and CsSPL9 were analyzed. Sequence analysis showed that the open reading frames of CsSPL6 and CsSPL9 are 1668 bp and 1116 bp, encoding 555 and 371 amino acids respectively. Both genes contain typical SBP domain and the recognition sites of microRNA156a. Quantitative real-time PCR analysis showed that the expression profile of Csn-miR156a was the highest in the buds, and the lowest in the 7th leaves, which was negatively correlated with CsSPL6 and CsSPL9. For different varieties(strains), the expression of Csn-miR156a was consistent with the growth of new shoots and the value of Photosynthesis index, but negatively correlated with the expression of CsSPL6 and CsSPL9, indicating. Csn-miR156a,CsSPL6 and CsSPL9 were involved in the growth of tea plant and can be used as markers to assess growth abilities among varieties. In addition, the expression of CsSPL9 was negatively correlatedwith Csn-miR156a under heat stress in four varieties (strains), which might be associated with the enhanced heat tolerance ability. The results showed that the expression of CsSPLs were negatively regulated by Csn-miR156a,which played an important role in the growth, development of tea plant and the stress resistance, providing a theoretical basis for understanding the growth and resistance mechanism of tea plant.关键词
茶树/生长势/高温/miR156a/SPLKey words
Camellia sinensis/growth potential/high temperature/miR156a/SPL分类
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刘亚芹,田坤红,孙琪璐,潘铖,李叶云,蒋家月,江昌俊..茶树miR156a靶基因SPL6和SPL9的克隆及表达分析[J].茶叶科学,2017,37(6):551-564,14.基金项目
茶树响应低温胁迫的 microRNA 发掘及其调控机制研究(31270729)、国家自然科学基金(31270729) （31270729）
