茶叶科学2017，Vol.37Issue(6)：565-574,10.
茶树Copine家族基因CsBON3的克隆与表达分析
Cloning and Expression Analysis of CsBON3 from Copine Gene Family in Tea Plant (Camellia sinensis)
摘要
Abstract
Copine protein, a kind of Ca2+-dependent protein or phospholipid-binding protein containing two C2 (N-terminal) and one vWA (C-terminal) conserved domain, plays an important role in intracellular signal transduction. Based on the sequence identity analysis, an EST sequence showing high homology to Copine family genes was selected from our previous tea plant transcriptome database. The sequence was verified to contain 1746 bp complete ORF, encoding 581 amino acids. Homologous alignment showed that the gene had the highest similarity with Arabidopsis AtBON3 (65%) and was named as CsBON3 (GenBank accession number: KY435900). Bioinformatic analysis showed that the molecular weight of CsBON3 protein was 63.66 kD and the theoretical isoelectric point was 5.48. It possesses the conserved domain unique to Copine family protein, which is a hydrophilic protein, no signal peptide site, non-secretory protein and no transmembrane domain. Expression analysis showed that CsBON3 had the highest expression levels in tea flowers and roots, and the lowest in stems and mature leaves. It was significantly up-regulated in tea plants treated with low temperature (4℃) for one day, and its expression was suppressed at dormant stage and up-regulated at growth stage. It was also up-regulated rapidly in the leaves with inoculation of Colletotrichum fructicola. The results indicated that the CsBON3 plays important roles in tea plant growth in response to low temperature and disease resistance.关键词
茶树/Copine家族/BON基因/逆境胁迫响应/休眠/表达分析Key words
tea plant/Copine family/BON gene/stress response/dormancy/expression analysis分类
农业科技引用本文复制引用
姚利娜,郝心愿,王璐,李娜娜,曾建明,杨亚军,王新超..茶树Copine家族基因CsBON3的克隆与表达分析[J].茶叶科学,2017,37(6):565-574,10.基金项目
国家自然科学基金(31370690)、国家茶叶产业技术体系(CARS-19)资助 （31370690）
