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基于UHPLC-Q-TOF/MS的不同产地普洱生茶化学成分差异研究

杨晨 戴伟东 吕美玲 李朋亮 刘栩 田军 万云龙 李继 林智

茶叶科学2017,Vol.37Issue(6):605-615,11.
茶叶科学2017,Vol.37Issue(6):605-615,11.

基于UHPLC-Q-TOF/MS的不同产地普洱生茶化学成分差异研究

Study on the Chemical Constituents of Pu-erh Teas from Different Areas by UHPLC-Q-TOF/MS

杨晨 1戴伟东 2吕美玲 3李朋亮 1刘栩 2田军 4万云龙 1李继 2林智3

作者信息

  • 1. 农业部茶树生物学与资源利用重点实验室
  • 2. 中国农业科学院茶叶研究所 浙江 杭州 310008
  • 3. 中国农业科学院研究生院,北京 100081
  • 4. 安捷伦科技(中国)有限公司,北京 100102
  • 折叠

摘要

Abstract

In order to fully understand the influences of the production area on pu-erh raw tea quality and chemical composition, 12 pu-erh raw tea samples were selected from different mountains (villages) in Lincang, Pu'er, and Xishuangbanna. The nonvolatile tea metabolite phenotypes of the pu-erh raw teas were analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry-four (UHPLC-Q-TOF/MS). The results showed that the pu-erh raw teas from different areas had distinct chemical compositions. Pu-erh raw teas from Xishuangbanna (including Mengla, Menghai, and Jinghong), Pu'er, and Lincang could be seperated by principal component analysis. Pu-erh raw teas could also be seperated according to producing areas (southeast, southwest, and northwest of pu-erh tea area). Seventy nine main components in pu-erh raw teas were identified, and the relationships between the components and the taste qualities of the 12 pu-erh raw teas were also analyzed. This study showed that the metabolite phenotypes analyzed by UHPLC-Q-TOF/MS could be used for the discrimination of pu-erh raw teas according to producing areas.

关键词

普洱生茶/产地/化学成分/液质联用/代谢组学/滋味

Key words

Pu-erh raw tea/area/chemical composition/LC-MS/metabolomics/taste

分类

轻工纺织

引用本文复制引用

杨晨,戴伟东,吕美玲,李朋亮,刘栩,田军,万云龙,李继,林智..基于UHPLC-Q-TOF/MS的不同产地普洱生茶化学成分差异研究[J].茶叶科学,2017,37(6):605-615,11.

基金项目

国家自然科学基金(31500561)、中央级公益性科研院所所级基本科研业务费专项(1610212016008)、现代农业产业技术体系建设专项资金(CARS-23) (31500561)

茶叶科学

OA北大核心CSCDCSTPCD

1000-369X

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