食品科学2017，Vol.38Issue(23)：87-93,7.DOI:10.7506/spkx1002-6630-201723015
刺葡萄皮醇提物和不同洗脱组分抗氧化成分及活性
Antioxidant Activity of Methanol Extract and Its Fractions from Spine Grape (Vitis davidii Fo?x.) Peel
摘要
Abstract
In order to evaluate the antioxidant activity and components of spine grape peel, crude extract from spine grape peel was prepared by ultrasonic assisted extraction with acidified methanol as the extraction solvent and separated by macroporous resin adsorption to obtain five main fractions (I–V). Spine grape peel showed high contents of total phenolics (1.836 mg/g mf), total flavonoids (0.874 mg/g mf), VC (3.567 mg/g mf), total condensed tannins (3.578 mg/g mf) and total anthocyanins (2.970 mg/g mf) . The antioxidant activity of the methanol extract as evaluated by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) assay and ferric reducing antioxidant power (FRAP) was 22.325, 17.595 and 26.487 μmol TE/g mf, respectively. Among 5 fractions, the water-eluted fraction I contained the highest contents of phenolics, flavonoids, VC, tannin and anthocyanins (0.876, 0.116, 1.577, 1.576 and 1.330 mg/g mf, respectively) and presented the best antioxidant activity (3.636, 10.109 and 13.415 μmol TE/g mf for DPPH radical scavenging activity, ABTS+· scavenging activity and FRAP, respectively). Significant correlations were observed between antioxidant activity and anthocyanins, tannin and VC contents, which suggested that these compounds might be the major antioxidants contributing to the observed activity. Therefore, spine grape peel is a promising source of antioxidants and can be used as a dietary supplement for health promotion.关键词
刺葡萄皮/抗氧化活性/活性成分Key words
spine grape peel/antioxidant activity/active components分类
轻工纺织引用本文复制引用
陆俊,张佳琦,罗丹,王珺,任艳艳,李忠海..刺葡萄皮醇提物和不同洗脱组分抗氧化成分及活性[J].食品科学,2017,38(23):87-93,7.基金项目
湖南省科技支撑计划项目(2015NK3022) （2015NK3022）
