食品科学2017，Vol.38Issue(23)：141-148,8.DOI:10.7506/spkx1002-6630-201723023
沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制
Anti-Aging Effect and Its Mechanism of Ethanol Extract from Sea Buckthorn Seed Meal in Caenorhabditis elegans
摘要
Abstract
In the present study, the anti-aging effect of sea buckthorn seed extracts (SBSE) obtained by ethanol extraction and macroporous resin purification was evaluated using Caenorhabditis elegans as a model organism and its underlying mechanism was discussed as well. The effect of different concentrations of SBSE was investigated on the lifespan, reproductive capability,heat and oxidative stress defense of C.elegans.In order to explore the anti-aging mechanism of SBSE at the gene level, the activity of antioxidant enzymes and the expression levels of related genes were determined. The results indicated that SBSE could significantly prolong the lifespan of C.elegans(P<0.05),and reduce the accumulation of lipofuscin.Meanwhile,the number of C.elegans offsprings treated with 0.05 mg/mL of SBSE(246.40 ± 21.11)was significantly bigger than the control (205.80 ± 20.42) (P < 0.05). SBSE could significantly enhance the heat and oxidative stress defense (P < 0.05). Furthermore, SBSE increased the activity of superoxide dismutase, catalase and glutathione peroxidase,down-regulated the pro-aging gene expression levels of age-1 and daf-2,and up-regulated the anti-aging gene expression levels of sir-2.1.Therefore,SBSE possess an anti-aging effect by increasing the activity of antioxidant enzymes and changing the expression of aging-related genes.关键词
沙棘粕醇提取物/秀丽隐杆线虫/抗氧化酶/抗衰老Key words
sea buckthorn seed extracts/Caenorhabditis elegans/antioxidant enzymes/anti-aging分类
轻工纺织引用本文复制引用
张佳婵,王昌涛,刘瑶,石秀芹,赵丹,李萌,王成涛,孙宝国..沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制[J].食品科学,2017,38(23):141-148,8.基金项目
国家自然科学基金面上项目(31571801) （31571801）
