食品科学2017，Vol.38Issue(23)：191-195,5.DOI:10.7506/spkx1002-6630-201723030
猪皮胶原多肽螯合钙增加大鼠骨密度
Calcium-Binding Collagen Polypeptide from Pigskin Gelatin Improves Bone Mineral Density in Rats
摘要
Abstract
In this paper, calcium-binding collagen polypeptide (CP-Ca) was synthesized by liquid-state reaction between collagen polypeptide prepared in our laboratory from pigskin gelatin and calcium gluconate, and the effect of CP-Ca on improving bone mineral density (BMD) in rats was investigated. Totally 120 female SD rats were equally divided into 10 groups: control group (deionized water by gavage), low-, middle- and high-dosage CP-Ca groups (139.28, 278.55 and 557.10 mg/(100 g·d)), low-, middle- and high-dosage CaCO3groups (25, 50 and 100 mg/(100 g·d)), and low-, middle- and high-dosage CP + CaCO3group (139.28 mg /(100 g·d) CP + 25 mg/(100 g·d) CaCO3, 278.55 mg/(100 g·d) CP + 50 mg/(100 g·d) CaCO3, 557.10 mg/(100 g·d) CP + 100 mg/(100 g·d) CaCO3). All rats were fed on a basic diet and deionized water. The body weight and length of the rats were recorded weekly. A three-day calcium metabolism experiment was carried out in the fourth week to estimate the apparent absorptivity of calcium. Serum calcium (S-Ca), serum phosphorusand (S-P), and alkaline phosphatese (ALP), BMD and bone calcium content were measured. The rat femurs were analyzed by scanning electron microscope (SEM). The results showed that CP-Ca at middle and high dosages and CP + CaCO3at high dosage could significantly promote body weight gain (P < 0.05), and the effect of CP-Ca was more apparent. CP-Ca and CP + CaCO3had obviously lower ALP activity than the control group (P < 0.05). CP-Ca and CP + CaCO3both at middle and high dosages significantly raised BMD compared to the control group (P < 0.05), and CP-Ca was more effective at the high dosage. At all dosages tested, CP-Ca and CP + CaCO3resulted in higher Ca bioavailability than CaCO3. CP-Ca could activate osteoblasts on the surface of bones, increase the density of bones and reduce bone calcium loss. Meanwhile, SEM revealed that CP-Ca and CP + CaCO3could prevent and improve osteoporosis caused by long-term calcium deficiency. CP-Ca was highly soluble, exhibited good dissociation ability, and was rich in calcium and polypeptides so than it can be used in functional foods and health products to prevent osteoporosis and improve BMD.关键词
猪皮明胶/多肽螯合钙/SD大鼠/骨密度Key words
pigskin gelatin/calcium-binding collagen polypeptide (CP-Ca)/SD rats/bone mineral density (BMD)分类
轻工纺织引用本文复制引用
许先猛,董文宾..猪皮胶原多肽螯合钙增加大鼠骨密度[J].食品科学,2017,38(23):191-195,5.基金项目
"十二五"国家科技支撑计划项目(2012BAD12B07-05) （2012BAD12B07-05）
