食品科学2017，Vol.38Issue(23)：196-200,5.DOI:10.7506/spkx1002-6630-201723031
基于尿苷二磷酸葡萄糖醛酸转移酶1解毒通路探讨枸杞汁对邻苯二甲酸二(2-乙基己基)酯代谢的干预作用
Intervention Effect of Lycium barbarum Juice on the Metabolism of Di-(2-ethylhexyl)phthalate Based on the Uridine Diphosphate Glucuronosyltransferase 1 Detoxification Pathway
摘要
Abstract
Purpose: This study aimed to explore the intervention effect of Lycium barbarum juice (LBJ) on the metabolism of di-(2-ethylhexyl) phthalate (DEHP) by analyzing the activity of uridine diphosphate glucuronosyltransferase 1 (UGT1) and metabolic detoxification profile in the liver of rats exposed to DEHP. Methods: Sixty Sprague Dawley male rats were randomly divided into three groups (n = 20 each): control group, DEHP group and LBJ group. The DEHP and LBJ groups were administered with normal saline and LBJ respectively by gavage once daily for 7 consecutive days after receiving a single dose of 3 000 mg/kg mb DEHP dissolved in sesame oil. The control group was treated with normal saline for 7 days after receiving the same volume of sesame oil of. Urine samples were collected for 24 hours daily during the experimental period. Totally 5 rats were sacrificed randomly in each group after 1, 3, 5 and 7 days of exposure. The activity of UGT1 was determined by an enzyme linked immunosorbent assay kit while the contents of mono-(2-ethylhexyl) phthalate (MEHP) in serum and urine and phthalic acid (PA) in urine were detected by high performance liquid chromatography. Results: The activity of UGT1 in the LBJ group significantly increased on the 5th day compared to the DEHP and control groups (P < 0.05).The content of serum MEHP in the LBJ group decreased significantly, whereas the contents of urine MEHP and PA increased compared to DEHP group. Conclusion: LBJ can promote the metabolism and excretion of DEHP through improving the activity of UGT1, making it a healthcare product or functional food against the potential hazard of phthalate esters (PAEs) or other poisonous chemicals.关键词
邻苯二甲酸二(2-乙基己基)酯/尿苷二磷酸葡萄糖醛酸转移酶1/枸杞汁/干预Key words
di-(2-ethylhexyl)phthalate(DEHP)/uridine diphosphate glucuronosyltransferase 1(UGT1)/Lycium barbarum juice (LBJ)/intervention分类
医药卫生引用本文复制引用
贺永健,黄少文,刘瑞菁,万发达,杨婕,刘焕,柳春红..基于尿苷二磷酸葡萄糖醛酸转移酶1解毒通路探讨枸杞汁对邻苯二甲酸二(2-乙基己基)酯代谢的干预作用[J].食品科学,2017,38(23):196-200,5.基金项目
农业部农产品质量安全监管项目(GJFP201501202) （GJFP201501202）
广东省科技计划项目(2013B020204001) （2013B020204001）
