食品科学2017，Vol.38Issue(23)：207-212,6.DOI:10.7506/spkx1002-6630-201723033
菠萝蜜多糖对脾淋巴细胞抗氧化作用及免疫功能的影响
Antioxidant and Immunoenhancing Activity of Polysaccharide from Artocarpus heterophyllus Lam.on Spleen Lymphocytes
摘要
Abstract
The antioxidant and immunomodulatory effects of water-soluble polysaccharide from jackfruit (Artocarpus heterophyllus Lam.) pulp (JFP-Ps) were investigated.The effect of JFP-Ps on the proliferation of normal mouse spleen lymphocytes, antioxidant capacity (total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activity) and malonic dialdehyde (MDA) contents, as well as the secretion of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and interleukin-1β (IL-1β) were detected by culturing mouse spleen lymphocytes in vitro in the presence of different concentrations of JFP-Ps. The results showed that JFP-Ps at 80 and 160 μg/mL significantly (P < 0.05) increased lymphocyte proliferation, and it at 40 μg/mL and above enhanced T-AOC, GSH-Px, SOD and CAT activities and reduced MDA content. Moreover, JFP-Ps at 40, 80 and 160 μg/mL significantly increased the secretion of TNF-α, IFN-γ and IL-1β in spleen lymphocytes (P < 0.05). These results indicate that JFP-Ps exerts immunomodulatory effect likely by inducing lymphocyte proliferation, enhancing antioxidant activity and increasing the secretion of TNF-α, IFN-γ and IL-1β.关键词
菠萝蜜多糖/脾淋巴细胞/抗氧化活性/细胞因子/免疫调节Key words
polysaccharide from jackfruit (Artocarpus heterophyllus Lam.)/spleen lymphocyte/antioxidant activity/cytokines/immune modulation分类
农业科技引用本文复制引用
朱科学,王颖倩,张彦军,贺书珍,徐飞,吴刚,谭乐和..菠萝蜜多糖对脾淋巴细胞抗氧化作用及免疫功能的影响[J].食品科学,2017,38(23):207-212,6.基金项目
海南省自然科学基金面上项目(317270) （317270）
南昌大学食品科学与技术国家重点实验室开放基金项目(SKLF-KF-201602) （SKLF-KF-201602）
