食品科学2017，Vol.38Issue(24)：1-7,7.DOI:10.7506/spkx1002-6630-201724001
乳酸菌细菌素Lac-B23对荧光假单胞菌及其生物膜的抑制作用
Anti-Biofilm and Antimicrobial Activity of Bacteriocin Lac-B23 from Lactic Acid Bacteria against Pseudomonas fluorescens
摘要
Abstract
The aim of this study was to elucidate the mode of action of a novel bacteriocin Lac-B23 from Lactobacillus plantarum B23,a small peptide with broad-spectrum antimicrobial activity,on Pseudomonas fluorescens.P.fluorescens was susceptible to bacteriocin Lac-B23 action, leading to the release of intracellular ATP and inorganic phosphate. Bacteriocin Lac-B23 first destroyed the extracellular membrane and then dissipated both transmembrane potential (Δφ) and transmembrane pH gradient (ΔpH), leading to the formation of channels in the cell membrane. Clear changes in the morphological integrity of P. fluorescens were observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The cell membrane was destroyed, leading to the death of cells. Bacteriocin Lac-B23 could affect the network structure of the biofilm produced by P.fluorescens and exhibited anti-biofilm activity.关键词
乳酸菌细菌素/荧光假单胞菌/生物膜/抑制Key words
lactic acid bacteria/bacteriocin/Pseudomonas fluorescens/biofilm/inhibition分类
轻工纺织引用本文复制引用
刘文婷,王伟,易华西,章检明,韩雪,张兰威,何胜华..乳酸菌细菌素Lac-B23对荧光假单胞菌及其生物膜的抑制作用[J].食品科学,2017,38(24):1-7,7.基金项目
国家自然科学基金面上项目(31571850 （）
31771988) （）
中国海洋大学"青年英才工程"项目(201712002) （201712002）
