食品科学2017，Vol.38Issue(24)：8-14,7.DOI:10.7506/spkx1002-6630-201724002
婴儿粪便长双歧杆菌的分离与多样性分析
Isolation and Diversity Analysis of Bifidobacterium longum from Infant Faeces
摘要
Abstract
The aim of this experiment was to study the diversity of Bifidobacterium longum isolated from infant feces. Using modified MRS medium, bifidobacterial strains were isolated from the feces of healthy infants in Harbin. To identify and analyze the diversity of B.longum strains,we used physiological and biochemical experiments,16S rDNA homology analysis, heat-shock protein 60 gene (hsp60) homology analysis, random amplified polymorphism DNA (RAPD) and multilocus sequence typing(MLST).Eighteen anaerobic strains were isolated,among which,7 were identified as B.longum subsp.longum,and 3 as B.longum subsp.infantis according to morphology,physical and biochemical properties,16S rDNA homology analysis and hsp60 homology analysis.The results of RAPD and MLST showed that the 7 strains of B.longum subsp.longum belonged to 6 genotypes while the 3 strains of B.longum subsp.infantis belonged to 2 genotypes.Overall, these results showed that there were differences in the diversity of B.longum isolated from infant feces.关键词
长双歧杆菌/热应激蛋白60基因(hsp60)/随机扩增多态性DNA(RAPD)/多位点序列分型(MLST)Key words
Bifidobacterium longum/heat-shock protein 60 gene(hsp60)/random amplified polymorphism DNA(RAPD)/multilocus sequence typing (MLST)分类
轻工纺织引用本文复制引用
张秋雪,刘晓婵,朱宗涛,万峰,孙思睿,贾芳芳,孟祥晨..婴儿粪便长双歧杆菌的分离与多样性分析[J].食品科学,2017,38(24):8-14,7.基金项目
国家自然科学基金面上项目(31671917) （31671917）
