食品科学2017，Vol.38Issue(24)：150-158,9.DOI:10.7506/spkx1002-6630-201724024
基于超高效液相色谱-串联质谱的橄榄果实多酚测定及其抗氧化活性分析
Determination of Polyphenols and Antioxidant Activity in Chinese Olive Fruits by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
The aims of the study were to identify and quantify the phenolic compounds present in extracts of Chinese olive fruit (Canarium album L.) and to investigate their respective antioxidant activities. A total of 12 phenolic compounds were identified in Chinese olive fruit by ultra-performance liquid chromatography (UPLC) coupled to time-of-flight mass spectrometry (TOF-MS). A quantitative method was established by UPLC coupled to triple quadrupole mass spectrometry (TQ-MS/MS) under the multiple reaction monitoring (MRM) mode. Phenolic compounds were separated within 15 min. The linearity (r > 0.98), limit of detection (LOQ) (< 1.436 ng/mL), limit of quantification (LOQ) (< 4.786 ng/mL), inter-and intra-day accuracy ( > 80.19%), precision ( > 1.12%) and recovery ( > 80.13%) of the method were all satisfactory and thus it could provide an efficient protocol to analyze phenolic compounds in fruit pulp extracts of Chinese olive. Quantitative analysis of different solvent extracts suggested that 3-O-galloylquinic acid and Geraniin isomers was the most abundant polyphenols in Chinese olive. Furthermore 80% acetone extract contained more polyphenols and possessed stronger antioxidant capacity.关键词
多酚/超高效液相色谱-串联质谱/橄榄Key words
phenolic compounds/ultra-performance liquid chromatography coupled to triple quadrupole mass spectrometry (UPLC-MS/MS)/Chinese olive分类
农业科技引用本文复制引用
常强,苏明华,陈清西,曾碧玉,李惠华,王伟..基于超高效液相色谱-串联质谱的橄榄果实多酚测定及其抗氧化活性分析[J].食品科学,2017,38(24):150-158,9.基金项目
厦门市科技计划资助项目(3502Z20142001) （3502Z20142001）
福建省科技重大专项(2013N002-4) （2013N002-4）
厦门市重大科技创新平台项目(3502Z20131004) （3502Z20131004）
福建省亚热带植物研究所基金项目(20150318-1) （20150318-1）
