食品科学2017，Vol.38Issue(24)：165-170,6.DOI:10.7506/spkx1002-6630-201724026
响应面法优化南极磷虾粗脂肪索氏提取工艺
Optimization of Soxhlet Extraction of Antarctic Krill Lipid by Response Surface Methodology
摘要
Abstract
The Soxhlet extraction of lipid from Antarctic krill was optimized using one-factor-at-a-time method and response surface methodology. The lipid yield was studied as a function of solvent type and composition, extraction temperature and time. The results showed that optimal extraction conditions that resulted in the maximum lipid yield of 12.32% were as follows: a mixture of hexane and ethanol (3.5:1, V/V) as extraction solvent, temperature 80 ℃, and time 5.4 h. Gas chromatography analysis showed that the lipid obtained was mainly composed of palmitic acid (C16:0, 28.86%), eicosapentaenoic acid (C20:5n3, 15.48%), oleic acid (C18:1n9c, 12.71%) and docosahexaenoic acid (C22:6, 12.22%). The unsaturated fatty acid content was above 57.47%, with monounsaturated fatty acids and polyunsaturated fatty acids accounting for 19.82% and 37.65%, respectively. Therefore, Antarctic krill is a novel source of polyunsaturated fatty acids, and has high nutritional value and could be widely used as dietary supplements.关键词
南极磷虾粗脂肪/脂肪酸/索氏提取法/响应面法Key words
Antarctic krill lipid/fatty acid/Soxhlet extraction/response surface methodology分类
轻工纺织引用本文复制引用
周莉,李佩璇,赵钰灵,曾晓雄..响应面法优化南极磷虾粗脂肪索氏提取工艺[J].食品科学,2017,38(24):165-170,6.基金项目
国家自然科学基金青年科学基金项目(NSFC31501521) （NSFC31501521）
江苏省自然科学基金青年基金项目(BK20140701) （BK20140701）
