食品科学2017，Vol.38Issue(24)：196-201,6.DOI:10.7506/spkx1002-6630-201724031
超高效液相色谱-四极杆/静电场轨道离子阱质谱测定植物提取物中的7种合成色素
Determination of 7 Artificial Dyes in Plant Extracts by Ultra-Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbit Trap High-Resolution Mass Spectrometry
摘要
Abstract
A rapid method was presented for the determination of 7 artificial dyes in plant extracts by ultra-performance liquid chromatography-electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-MS). Samples were extracted with water, and the extract was cleaned up on a polyamide solid phase extraction (SPE) column and separated by UPLC on a C18column using a mobile phase consisting of 5 mmol/L ammonium acetate-acetonitrile with gradient elution. Data were collected by ESI Q-Orbitrap MS. The results showed that good linearity was observed in the concentration range from 10 to 500 μg/mL for all 7 artificial dyes, with correlation coefficients (r2) greater than 0.99. The limits of detection (LODs) ranged from 0.001 2 to 0.052 mg/kg. The average recoveries were in the range from 60.8% to 91.8% at 3 spiked levels with relative standards deviation (RSDs) between 2.1% and 6.5%. This method proved to be simple, time saving, accurate, reliable and sensitive and thus can be used for the fast screening and confirmation of artificial dyes in plant extracts.关键词
植物提取物/合成色素/超高效液相色谱-四极杆/静电场轨道离子阱质谱/高分辨质谱Key words
plant extracts/artificial dyes/ultra-performance liquid chromatography-quadrupole/electrostatic field orbit trap mass spectrometry/high-resolution mass spectrometry分类
轻工纺织引用本文复制引用
朱振宝,张亚莉,贾玮,孔祥虹,何强..超高效液相色谱-四极杆/静电场轨道离子阱质谱测定植物提取物中的7种合成色素[J].食品科学,2017,38(24):196-201,6.基金项目
国家自然科学基金面上项目(31671888) （31671888）
陕西省自然科学基金面上项目(2015JM3085) （2015JM3085）
