食品科学2017，Vol.38Issue(24)：213-217,5.DOI:10.7506/spkx1002-6630-201724034
固相萃取-气相色谱法测定黄酒中β-苯乙醇含量
Determination of β-Phenylethanol in Rice Wine by Solid Phase Extraction-Gas Chromatography
摘要
Abstract
Objective: To establish a method for the determination of β-phenylethanol in rice wine by solid phase extraction (SPE)-gas chromatography (GC). Methods: β-Phenylethanol was enriched from 3.0 mL of rice wine with an activated and balanced HLB solid phase extraction column, which was washed with 3.0 mL of water and 3.0 mL of methanol-water (4:6, V/V),and eluted with 3.0 mL of methanol.A gas chromatograph equipped with flame ionization detector(GC-FID)was used to analyze the target compounds. Results: In the concentration range of 10.1–202.0 mg/L, good linearity was achieved with a correlation coefficient (r) of 0.999 9. The limit of detection (LOD, RSN= 3) and the limit of quantification (LOQ, RSN= 10) were 0.9 and 3.0 mg/L, respectively. The recovery of β-phenylethanol was in the range of 95.5%–98.5%. The precision values expressed as relative standard deviations (RSDs) were 1.1%–1.4%. Conclusion: The method is simple, convenient, accurate and applicable to the determination of β-phenylethanol in rice wine and can effectively reduce the pollution of the gas chromatography system by rice wine matrix and prevent water in rice wine samples from bringing harm to the capillary column.关键词
固相萃取/气相色谱/黄酒/β-苯乙醇Key words
solid phase extraction (SPE)/gas chromatography (GC)/rice wine/β-phenylethanol分类
轻工纺织引用本文复制引用
芦智远,张耀武,李雪娟,冯歆轶,薛慧慧,郭松年,刘佳..固相萃取-气相色谱法测定黄酒中β-苯乙醇含量[J].食品科学,2017,38(24):213-217,5.基金项目
国家质量监督检验检疫总局科技项目(2016QK109) （2016QK109）
