食品科学2017，Vol.38Issue(24)：265-271,7.DOI:10.7506/spkx1002-6630-201724043
固相萃取-超高效液相色谱-串联质谱法同时测定酸奶中55种塑料添加剂
Simultaneous Determination of 55 Plastic Additives Migrating from Plastic Containers to Yogurt by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method has been developed to determine 55 plastic additives including antioxidants, plasticizers, ultraviolet light absorbers in yogurt by solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS). Samples were extracted with acetonitrile and the extract was then cleaned up on an HLB SPE column. The UPLC system equipped with a ACQUITY UPLC BEH C18analytical column (2.1 mm × 50 mm, 1.7 μm) was operated utilizing methanol-0.1% formic acid as the mobile phase in gradient elution mode. Electrospray ionization in positive ion mode (ESI+) with multiple reaction monitoring (MRM) was used, and the analytes were quantified by the external standard method. The calibration curves displayed good linearity in a wide concentration range with a correlation coefficient of over 0.97. The average recoveries from spiked yogurt at three concentrations ranged from 62.4% to 93.5% with relative standard deviations (RSD) varying from 2.4% to 13.4%. The limits of detection (LOD) were 0.2–40 μg/kg. Twenty-three plastic additives at concentrations from 7.0 to 39 μg/kg were detected in 20 samples by the proposed method. This method can simultaneously identify and quantify a wide variety of plastic additive compounds with distinctive physicochemical properties in yogurt with high sensitivity.关键词
塑料添加剂/超高效液相色谱-串联质谱/高通量检测/酸奶Key words
plastic additives/ultra performance liquid chromatography-tandem mass spectrometry/high throughput detection/yogurt分类
化学化工引用本文复制引用
薄海波,星玉秀,吉生军,王静莹,杨娟..固相萃取-超高效液相色谱-串联质谱法同时测定酸奶中55种塑料添加剂[J].食品科学,2017,38(24):265-271,7.基金项目
青海省应用基础研究项目(2014-ZJ-722) （2014-ZJ-722）
