响应面试验优化QuEChERS法提取鸡蛋中杂色曲霉毒素工艺及方法学验证OA北大核心CSCDCSTPCD
Optimization and Validation of QuEChERS Extraction for Determination of Sterigmatocystin in Eggs by Response Surface Methodology
使用乙腈溶液对鸡蛋中杂色曲霉毒素进行提取,后加入无水Na2SO4、NaCl和无水CH3COONa进行盐析,取出上层乙腈后加入C18吸附剂和无水Na2SO4进行净化浓缩后上机检测.采用Plackett-Burman试验、单因素试验和响应面法优化,最大限度地提高杂色曲霉毒素的提取率.使用基质匹配曲线外标法定量,所有阳性样品均使用免疫亲和柱法复测.结果表明,杂色曲霉毒素在0.125~1000ng/mL质量浓度范围内线性良好,相关系数达0.9996,检出…查看全部>>
A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure was presented andoptimized for the determination of sterigmatocystin in eggs by high performance liquid chromatography-mass spectrometry (HPLC-MS). The analyte was extracted from samples with aqueous acetonitrile solution, followed by salting out using anhydrous NaSO4, NaCl and CH3COONa,and then purification and concentration with C18sorbent and anhydrous Na2SO4before analysis.…查看全部>>
周健;许娇娇;金米聪;蔡增轩;黄百芬;任一平
浙江省疾病预防控制中心理化毒理所,浙江 杭州 310051宁波市疾病预防控制中心,浙江省微量有毒化学物健康风险评估技术研究重点实验室,浙江 宁波 315010浙江省疾病预防控制中心理化毒理所,浙江 杭州 310051宁波市疾病预防控制中心,浙江省微量有毒化学物健康风险评估技术研究重点实验室,浙江 宁波 315010浙江省疾病预防控制中心理化毒理所,浙江 杭州 310051浙江省疾病预防控制中心理化毒理所,浙江 杭州 310051
化学化工
鸡蛋杂色曲霉毒素QuEChERS法响应面法超高效液相色谱-质谱联用
eggsterigmatocystinQuEChERS extractionresponse surface methodologyhigh performance liquid chromatography-mass spectrometry
《食品科学》 2017 (24)
288-295,8
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