肉类研究2017，Vol.31Issue(12)：36-40,5.DOI:10.7506/rlyj1001-8123-201712007
加速溶剂萃取-高效液相色谱-荧光检测法测定哈尔滨红肠中的4种多环芳烃
Detection of Four Polycyclic Aromatic Hydrocarbons in Harbin Red Sausage by Accelerated Solvent Extraction Coupled with High Performance Liquid Chromatography with Fluorescence Detection
摘要
Abstract
A quantitative analysis method for four polycyclic aromatic hydrocarbons (PAHs) in Harbin red sausage was established by accelerated solvent extraction coupled with high performance liquid chromatography with fluorescence detection (ASE-HPLC-FLD). The extraction and chromatographic conditions were optimized and the quantification was carried out by the external standard method. The results showed that the calibration curves for all four PAHs, benzo (a) anthracene (B(a)A), chrysene (CHR), benzo(b) fluoranthene (B(b)F), benzo (a) pyrene (B(a)P), were linear with correlation coefficient (R2) greater than 0.999 0. The detection limits for the PAHs were 1.016, 1.872, 0.428 and 0.104 μg/kg, respectively. Overall recoveries from spiked samples were between 50% and 90% with relative standard deviation (RSD) below 8%. All the PAHs were detected simultaneously, sensitively, conveniently and accurately with high reproducibility by this method, making it useful for rapid determination of PAHs in Harbin red sausages.关键词
哈尔滨红肠/多环芳烃/高效液相色谱/荧光检测Key words
Harbin red sausage/polycyclic aromatic hydrocarbons (PAHs)/high performance liquid chromatography (HPLC)/fluorescence detection分类
医药卫生引用本文复制引用
李聪,赵颖,徐宝才,顾千辉..加速溶剂萃取-高效液相色谱-荧光检测法测定哈尔滨红肠中的4种多环芳烃[J].肉类研究,2017,31(12):36-40,5.基金项目
江苏省重点研发计划(现代农业)项目(BE2016306) （现代农业）
