食品与机械2017，Vol.33Issue(8)：23-26,154,5.DOI:10.13652/j.issn.1003-5788.2017.08.006
酿酒酵母低渗敏感性突变株的选育
Breeding of low-permeability sensitive mutant from Saccharomyces cerevisiae
摘要
Abstract
In order to improve the efficient secretion of intracellular product in Saccharomyces cerevisiae,wild diploid Y1 (2n) were mutagenized with UV treatment as original strain.To further identify.the degree of autolysis of each mutant strain was contrasted in low permeability condition by the test of its content of FDP,extracellular nucleic acids and proteins.The results showed:Hs5 * (n) showed the highest degree of conditional autolysis in low permeability condition,the concentration of FDP in extracellular environment was 25.81 μg/mL.At the sixth hour in low permeability,its penetration rate of nucleic acid and protein in extracellular environment were 1.86 and 2.07,respectively.In addition,mutant strain Hs2 * (n),Hs1 (2n)also had a great extent of conditional autolysis capacity.These three low-permeability mutant strains obtained in this study possessed greater efficiency of intracellular product secretion contrast with wild strain.关键词
酿酒酵母/紫外诱变/低渗敏感性突变株/条件性自溶Key words
Saccharomyces cerevisiae/UV mutagenesis/low-permeability mutant/conditional autolysis引用本文复制引用
叶菁,岳希洁,蒋雪薇,罗晓明,陈代文,吴健,李赤翎..酿酒酵母低渗敏感性突变株的选育[J].食品与机械,2017,33(8):23-26,154,5.基金项目
长沙市科技计划重点项目(编号:K1403029-11) （编号:K1403029-11）
清远市科技计划项目(编号:2013A024,2014A023) （编号:2013A024,2014A023）
湖南省水生资源食品加工工程技术研究中心开放基金项目(编号:2015GCZX07) （编号:2015GCZX07）
