食品与机械2017，Vol.33Issue(8)：27-30,4.DOI:10.13652/j.issn.1003-5788.2017.08.007
产银杏内酯内生真菌的分离与鉴定
Isolation and identification of ginkolides-producing endophytic fungi from Ginkgo biloba Linn.
摘要
Abstract
In the present study,the method of tissue culturing was used to isolate the endophytic fungi from healthy ginkgo roots and stems.After repeated separation and purification.116 Ginkgo endophytes were isolated.Metabolites of these fungi were preliminarily analyzed by high-performance liquid chromatography (HPLC),and a ginkgolide producing endophyte strain was finally screened out.The production of ginkgolides by this strain was more than 11.4 mg/L.The endophytic fungi strain was identified as Mucor spp.by morphology characteristics and phylogenetic analysis of internal transcribed spacer (ITS),and It was the first report in the ginkgolides producing endophytic fungus from Ginkgo biloba.关键词
银杏/内生真菌/内酯类化合物/鉴定Key words
Ginkgo biloba/endophytes/ginkgolides/identification引用本文复制引用
陈小明,何福林,李志忠,袁志辉..产银杏内酯内生真菌的分离与鉴定[J].食品与机械,2017,33(8):27-30,4.基金项目
湖南省教育厅科研项目(编号:15C0585) （编号:15C0585）
湘南优势植物资源综合利用湖南省重点实验室开放基金项目(编号:XNZW15C11) （编号:XNZW15C11）
