食品与机械2017，Vol.33Issue(8)：150-154,5.DOI:10.13652/j.issn.1003-5788.2017.08.033
不同极性柳叶蜡梅叶萃取物总酚含量及其抗氧化、抑菌能力研究
Study on total polyphenol content and antioxidant, antimicrobial activities of different polarity fractions of Chimonanthus salicifolicus Hu leaves
摘要
Abstract
Chimonanthus salicifolicus Hu leaves extracts were prepared by methanol circumfluence extraction.Five fractions were obtained by extraction with different polar solvents including n-hexane.dichloromethane,ethyl acetate,n butanol and water.Then fractions were detected by total polyphenol content,reducing power,scavenging DPPH free radical and antimicrobial activities.The results showed that all the fractions exhibited high content of total polyphenol,and ethyl acetate fraction had the highest phenolic compounds,with the content of (309.50±4.81) mg/g.The fractions had strong antioxidative capacities such as reducing power and DPPH free radical scavenging ability.The fractions of methanol,n-hexane,dichloromethane,ethyl acetate,n-butanol and water had certain scavenging ability on DPPH free radical with IC50 of 1.24,1.11,0.61,0.48,0.98 and 1.36 mg/mL,respectively.The fractions also had the inhibiting effects on E.coli,S.aureus,B.subtilis and P.aeruginosa.Dichloromethane fraction presented the best antimicrobial effect than other fractions.Its MIC against four common bacterial strains was 2 mg/mL.In conclusion,the fractions have good antioxidant and antimicrobial activities,and this research was profitable for the further study of the separation of active compounds from Chimonanthus salicifolicus Hu leaves.关键词
柳叶腊梅叶/极性/萃取物/抗氧化/抑菌Key words
Chimonanthus salicifolicus Hu leaves/different polarity fractions/antioxidant/antimicrobial activity引用本文复制引用
吴永祥,王祥,江海涛,丁康俊,赵成龙,胡长玉..不同极性柳叶蜡梅叶萃取物总酚含量及其抗氧化、抑菌能力研究[J].食品与机械,2017,33(8):150-154,5.基金项目
安徽省高校自然科学研究重点项目(编号:KJ2017A398) （编号:KJ2017A398）
2016年度安徽省大学生创新创业训练计划项目(编号:AH201610375055) （编号:AH201610375055）
博士人才引进启动基金项目(编号:2016xkjq004) （编号:2016xkjq004）
