中国食品添加剂Issue(12)：131-135,5.
组成型壳聚糖酶制备壳寡糖酶解条件的优化
Optimization of enzymatic hydrolysis conditions for chitooligosaccharides with constitutive chitosanase
李燕 1赵华 1李佳 1王宇鹏1
作者信息
- 1. 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457
- 折叠
摘要
Abstract
In this paper,the preparation of chitooligosaccharides by chitosan hydrolyzed with constitutive chitosanase was studied.The chitooligosaccharides yield (mg)was the index,and hydrolysis pH,hydrolysis temperature (℃),substrate concentration (％)and hydrolysis time (min)were independent variables.On the basis of single factor experiment,the optimum hydrolysis process was determined by orthogonal experiment and the best conditions were:hydrolysis pH 7,hydrolysis temperature 50℃,substrate concentration 1％,hydrolysis degradation time 90 min,the production of chitooligosaccharide was 5.476 mg.关键词
组成型壳聚糖酶/酶解/壳聚糖/壳寡糖。Key words
constitutive chitosanase/hydrolysis/chitosan/chitooligosaccharides分类
生物科学引用本文复制引用
李燕,赵华,李佳,王宇鹏..组成型壳聚糖酶制备壳寡糖酶解条件的优化[J].中国食品添加剂,2017,(12):131-135,5.
