现代食品科技2017，Vol.33Issue(12)：6-15,10.DOI:10.13982/j.mfst.1673-9078.2017.12.002
6-姜烯酚对H2O2诱导NCM460和HCT116氧化损伤的作用研究
Effects of 6-shogaol on H2O2-Induced Oxidative Damage in NCM460 and HCT116
摘要
Abstract
In this study,the model of oxidative damage in colorectal cancer cells was established.After the stimulation of hydrogen peroxide (H2O2),the different effects and possible molecular mechanisms of 6-shogoal on H2O2-induced oxidative damage in human intestinal epithelial cells (NCM460) and in situ colon cancer cells (HCT116) cells in vitro were investigated.Inverted microscope was used to observe the changes of cell morphology in HCT116 and NCM460 induced by H2O2 with different concentrations of6-shogoal.CCK-8 (Cell Counting Kit-8) method was used to screen the concentration interval of 6-shogaol and the cell survival rate was determined.Annexin and V-FITC/PI cytometry were used to detect apoptosis of different groups.The expressions of related apoptotic proteins (Caspase-3,poly (ADP-ribose) polymerase-1 (PARP-1),MCC1,A2F,B-cell lymphoma-2 (Bcl-2)) were detected by Western-blot.As compared with the model group,6-shogaol could reduce the expressions of caspase-3,PARP-1,MCC-1,A2F and promote the expression of Bcl-2 (p ＜ 0.05) in NCM460 induced by H2O2,indicating that 6-shogaol had anti-oxidant and pro-proliferative effects (p＜0.05).However,6-shogoal could promote the expression of caspase3,PARP-1,MCC1 and A2F,and inhibit the expression of Bcl-2 (p＜0.05) in HCT116 induced by H2O2,indicating that 6-shogaol could enhance the oxidative damage of HCT116 induced by H2O2 and inhibit cell proliferation (p＜0.05).It could be concluded that 6-shogaol had different opposite effects in NCM460 and HCT116 induced by H2O2,which might provide guidance for further studies on specific mechanisms or pathways of 6-shogoal against colorectal cancer.关键词
过氧化氢(H2O2)/人正常肠上皮细胞(NCM 460)/原位结肠癌细胞(HCT116)/6-姜烯酚/细胞增殖Key words
hydrogen peroxide (H2O2)/human intestinal epithelial cells (NCM460)/in situ colon cancer cells (HCT116)/6-shogoal/cell proliferation引用本文复制引用
王宇锋,陈超,杨银学,巩发海,孙书豪,李海..6-姜烯酚对H2O2诱导NCM460和HCT116氧化损伤的作用研究[J].现代食品科技,2017,33(12):6-15,10.基金项目
国家自然科学基金项目(81460369) （81460369）
宁夏自然科学基金项目(NZ16136) （NZ16136）
2017年宁夏“研究生教育创新计划”学位点建设项目(YXW2017014) （YXW2017014）
