现代食品科技2017，Vol.33Issue(12)：38-43,6.DOI:10.13982/j.mfst.1673-9078.2017.12.006
大枣多糖抗氧化及抗炎活性的研究
Antioxidant and Anti-inflammatory Activities of Polysaccharide from Zizyphus jujuba Mill
摘要
Abstract
In this study,antioxidant activity of a jujube polysaccharide from Zizyphusjujuba Mill was investigated by establishing 4 kinds of in vitro models,including 1,1-diphenyl-2-picrylhydrazyl (DPPH),2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS),and hydroxyl radical (·OH) scavenging activities as well as reducing power assay.The model of macrophage RAW264.7 induced by lipopolysaccharide (LPS) was established to study the anti-inflammatory activity of jujube polysaccharide.The results showed that jujube polysaccharide had strong antioxidant activity and the IC50 of jujube polysaccharide on the DPPH·,ABTS and ·OH free radicals were 0.9,2.8 and 1.1 mg/mL,respectively.The antioxidant activity of jujube polysaccharide exhibited a dose-dependent manner.When the value of total reducing power reached 1.0,the corresponding Vc and jujube polysaccharide concentrations were 0.08 mg/mL and 2.95 mg/mL,respectively.High doses of jujube polysaccharide could significantly reduce the levels of inflammatory cytokines including cyclooxygenase-2 (COX-2),tumor necrosis factor-alpha (TNF-a) and interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) in RAW264.7 cells,which showed that jujube polysaccharide had strong anti-inflammatory activity.Jujube polysaccharide was expected as an antioxidant and anti-inflammatory agents used in functional food and medicine industry.关键词
大枣/多糖/自由基/抗氧化活性/抗炎活性Key words
Zizyphus jujuba Mill./polysaccharide/free radicals/antioxidant activity/anti-inflammatory activity引用本文复制引用
展锐,邵金辉..大枣多糖抗氧化及抗炎活性的研究[J].现代食品科技,2017,33(12):38-43,6.基金项目
新疆农业科学院青年基金项目(xjnkq-2014027) （xjnkq-2014027）
