食品与发酵工业2017，Vol.43Issue(12)：199-204,6.DOI:10.13995/j.cnki.11-1802/ts.015034
超高效合相色谱法测定火龙果果酒中游离氨基酸的含量
Determination of free amino acids in pitaya wine by ultra performance convergence chromatography
摘要
Abstract
A new method based on ultra performance convergence chromatography (UPC2) was developed for analysis of free amino acids in fruit wine.They were separated on an Acquity UPC2 Torus Diol (3.0 mm × 100 mm,1.7 μm) with a mobile phase consisted of supercritical CO2 and methanol ∶ acetonitrile (1∶1 v/v,with 2.5 mmol/L ammonium acetate as mobile phase modifier).The flow rate was 2.0 mL/min and DAD detection was set at 280 nm with a compensation range of 330-430 nm.Back pressure was 1 800 psi.The results showed good linearity (R2 =0.999 0-0.999 8) and high resolution (＞ 1.22).The limits of detection (LOD) and quantification (LOQ) ranged from 23-57 ng/L and 70-178 ng/L,respectively.The linear range was between 20 and 400 μg/mL.The spiked recoveries were ranged from 85.6％ to 101.12％.It was applied for the free amino acids analysis of selected pitaya wine,which could also provide a new method for the analysis of free amino acid distribution in other food matrix from different sources.关键词
超高效合相色谱(UPC2)/游离氨基酸/测定/火龙果果酒Key words
ultra performance convergence chromatography (UPC2)/free amino acids/determination/pitaya wine引用本文复制引用
齐宁利,龚霄,马丽娜,张苏慧,龙倩倩,李积华..超高效合相色谱法测定火龙果果酒中游离氨基酸的含量[J].食品与发酵工业,2017,43(12):199-204,6.基金项目
中国热带农业科学院基本科研业务费专项资金(1630122017021) （1630122017021）
湛江市科技计划项目(2016A03012) （2016A03012）
农业部公益性行业(农业)科研专项(201303077) （农业）
