摘要
Abstract
In this paper,a chemiluminescence immunoassay (CLIA) for simultaneously detecting 6 β-agonist drugs residues in animal-derived food was established.The limits of detection (LODs) for clenbuterol,cimaterol,bromobuterol,bambuterol,mabuterol and sibuterol in pork were 0.48,1.96,0.41,0.29,0.42 and 0.87 μg/kg,respectively,and in mutton were 0.37,1.78,0.43,0.38,0.31 and 0.96 μg/kg,respectively.Mean recoveries from spiked samples ranged from 66.7％ to 106.4％,and the intra-assay and inter-assay relative standard deviation (RSD) values were less than 15％.The half maximal inhibitory concentration (IC50) of cimaterol was 0.254 μg/L under optimum conditions,and its cross-reaction rates with clenbuterol,bromobuterol,bambuterol,mabuterol and sibuterol were 438.0％,470.0％,434.0％,561.0％ and 198.0％,respectively.The developed immunoassay was more accurate and reliable than liquid chromatographic-tandem mass spectrometric (LC-MS/MS).Therefore,chemiluminescence immunoassay proved to be an effective analytical technique for monitoring β-agonist drug residues in animal-derived food owing to its high sensitivity.关键词
化学发光免疫分析/动物性食品/β-受体激动剂Key words
chemiluminescence immunoassay/animal-derived food/β-agonist分类
农业科技
