食品工业科技2017，Vol.38Issue(21)：37-41,5.DOI:10.13386/j.issn1002-0306.2017.21.008
垫状卷柏总黄酮的抗氧化和抗肿瘤活性
Antioxidant and antiproliferative activities of total flavonoids extracted from Selaginella pulvinata (Hook, et Grev.) Maxim
摘要
Abstract
Objective:To explore the antioxidant activity of Selaginella pulvinata(Hook,et Grey.)Maxim of total flavone and its growth inhibitory effect on HeLla、PC-3M and MDA-MB231 cells.Methods:Fenton reaction,pyrogallol auto-oxidation and DPPH radical-scavenging systems were used for antioxidant evaluation of total flavonoids in vitro.The reducing power was evaluated by potassium ferricyanide assay.Growth inhibitory effect of total flavonoids on Hela,PC-3M and MDA-MB231 cells was assessed by CCK-8 assay.Results:The scavenging effects of the total flavonoids on the hydroxyl,superoxide anion and DPPH free radicals increased as the total flavonoids concentration increased within 20～100 μg/mL.The EC50 of total flavonoids in scavenging hydroxyl,superoxide anion and DPPH free radicals were 111.86,89.24,26.51 μg/mL,respectively.The growth of HeLa,PC-3M and MDA-MB231 cells was remarkably inhibited by total flavonoids in a dose-dependent manner within the conservation of total flavonoids of 6.59～79.02 μg/mL.The inhibiting Hela,PC-3m and MDA-MB231 cells growth in 72 h was higher than in 48 h.Conclusion:Total flavonoids of Selaginella pulvinata (Hook,et Grey.) Maxim shows strong antioxidant activity and good inhibitory effect against the growth of Hela,PC-3m and MDA-MB231 cells.关键词
垫状卷柏/总黄酮/抗氧化/肿瘤细胞/生长抑制Key words
Selaginella pulvinatam Maxim/total flavorne/antioxidant/tumor cells/growth inhibition分类
轻工纺织引用本文复制引用
郑瑞凤,刘嵬,梁立,杨晨,颜军,苟小军,何钢..垫状卷柏总黄酮的抗氧化和抗肿瘤活性[J].食品工业科技,2017,38(21):37-41,5.基金项目
四川省科技厅应用基础项目(2015JY0117) （2015JY0117）
四川省教育厅基础项目(17ZA0089) （17ZA0089）
四川省高校重点实验室项目(TRCWYXFZCH2016014). （TRCWYXFZCH2016014）
