食品工业科技2017，Vol.38Issue(21)：151-156,161,7.DOI:10.13386/j.issn1002-0306.2017.21.031
橡子粉酶法制备低聚异麦芽糖的工艺研究
Study on the preparation of isomalto-oligosaccharide by enzymatic methods of acorn starch
摘要
Abstract
With the acorn starch as raw material,the process of preparation of isomalto-oligosaccharide by enzymatic methods were optimized in order to increase the content of isomaltose,panose and isomaltotriose of isomalto-oligosaccharide.Box-Benhnken response surface analysis method was used to optimize the process conditions of liquefying acorn starch with thermostable α-amylase.The results showed that the DE value of 13％ as the best liquefaction index,the optimal liquefaction conditions were 31 rin of liquefaction time,95 ℃ of liquefaction temperature,6.6 of liquefying pH value,13 U/g of thermostable α-amylase,at this point,in combination with the production practice,the value of the liquefied liquid DE was 12.91％,then through single factor experiments,the process conditions of saccharifying acorn starch liquefying liquor with pullulanase and glucoamylase were investigated,the saccharification conditions were optimized to be 25 U/g of pullulanase,130 U/g of glucoamylase,60 ℃ of saccharification temperature and 10 h of saccharification time,at this optimal condition,the content of(IG2 + P + IG3)was predicted to 36.11％ ± 0.17％,the optimum concentration of α-glucosidase in transglycosylation process was 1.5 U/g,under the optimal condition,the total content of isomaltose,panose,and isomaltotriose were 36.27％ ± 0.18％.关键词
低聚异麦芽糖/橡子粉/液化/糖化/转苷/工艺Key words
isomalto-oligosaccharide/acorn starch/liquefaction/saccharification/transglycosylation/technology分类
轻工纺织引用本文复制引用
张智,李晴,化洪苓,尹文哲,李杰,马建章..橡子粉酶法制备低聚异麦芽糖的工艺研究[J].食品工业科技,2017,38(21):151-156,161,7.基金项目
大熊猫益生菌制剂的研究(SG1409) （SG1409）
黑龙江省应用技术研究与开发计划项目(GA15B203). （GA15B203）
