食品工业科技2017，Vol.38Issue(22)：140-144,162,6.DOI:10.13386/j.issn1002-0306.2017.22.028
伊犁地区肉牛源产志贺毒素大肠杆菌的血清型和ERIC-PCR基因型研究
Study on serotype and ERIC-PCR typing of Shiga toxin-producing Escherichia coli in Yili area
摘要
Abstract
Objective:To investigate the food contamination and genetic diversity of the Shiga toxin producing Escherichia coli in cattle breeding and processing environment in Yili area.To provide the basic data for risk monitoring and control in the industrial chain of pathogenicity E.coli associated foodborne disease.Methods:According to the national standard and the polymerase chain reaction (PCR) method detected the contamination of Shiga toxin-producing E.coli in the 553 samples including the breeding link forage and faeces,slaughter link.The isolation and identification STEC strains were used to detect the 7 common serotypes(O145,O157,O45,O103,O111,O26,O121) with PCR methods and ERIC-PCR genotyping.Results:There were 39 strains encoding Shiga toxin gene in 553 samples.The detection rate of STEC was about 7.1％.The detection of common serotype PCR have 2 strains and 5 strains in the serotype O111 and O145,the detection rate was about 5.1％ and 12.8％,respectively.ERIC-PCR genotyping of STEC had 10 genetic subtypes and divided into 3 clusters.There were 23 strains in A cluster,the similarity was from59％ to 100％,which indicated that the relationship among these strains was relatively close.Conclusion:The beef cattle feces would be the source of Shiga toxin-producing E.coli and the genetic relationship of these strains was close in Yili area.关键词
伊犁地区肉牛/产志贺毒素大肠杆菌/PCR检测/ERIC-PCR基因分型Key words
beef cattle in Yili area/Shiga toxin-producing Escherichia coli/PCR method/ERIC-PCR genetyping分类
轻工纺织引用本文复制引用
刘英玉,张妍,夏利宁,姚刚,张晓红..伊犁地区肉牛源产志贺毒素大肠杆菌的血清型和ERIC-PCR基因型研究[J].食品工业科技,2017,38(22):140-144,162,6.基金项目
新疆维吾尔自治区自然科学基金(2014211B020) （2014211B020）
自治区博士后普通资助. （）
