食品工业科技2018，Vol.39Issue(1)：29-33,5.DOI:10.13386/j.issn1002-0306.2018.01.006
黑枸杞提取物对HBV的抑制作用及抗氧化能力测定
The inhibitory effect of black wolf berry extracts on HBV and the capacity of its antioxidant
摘要
Abstract
Objective:To study the effect of black wolfoerry extracts against hepatitis B virus and to detect its antioxidant activity.Methods:HepG22.2.15 cells were cultured in vitro,MTT was used to detect the cytotoxicity of black wolfberry extracts against HepG2 2.2.15 cells.The expression of HBsAg and HBeAg in the culture medium of HepG2 2.2.15 cells was detected by double antibody sandwich method.To determine the reducing prower and scavenging capacity of hydroxyl radical and DPPH free radical of different concentration of black wolfberry.Resuhs:MTT assay showed no obvious cytotoxicity of black wolfberry extracts on HepG2 2.2.15 cells.Compared with the negative control group,black wolfberry extracts had inhibitory effects on the expression of HBsAg and HBeAg in HepG2 2.2.15 cells(p ＜ 0.05).And with the increase of the concentration of black woltberry extracts,its abilities of reducing and scavenging hydroxyl radical,scavenging DPPH free radical (DPPH·) were also gradually enhanced.Conclusion:black wolfberry extracts have inhibitory effect on hepatitis B virus,and have a good antioxidant capacity.关键词
黑枸杞/HepG22.2.15细胞/HBsAg/HBeAg/抗氧化能力Key words
black wolfberry/HepG2 2.2.15 cells/HBsAg/HBeAg/antioxidant capacity分类
轻工纺织引用本文复制引用
高恩怡,龚红菲,张艳群,曾永联,杨扬,梁成钦,韦京辰..黑枸杞提取物对HBV的抑制作用及抗氧化能力测定[J].食品工业科技,2018,39(1):29-33,5.基金项目
广西科学基金(2016GXNSFAA380041) （2016GXNSFAA380041）
国家自然科学基金(81560574). （81560574）
