食品工业科技2018，Vol.39Issue(1)：46-51,6.DOI:10.13386/j.issn1002-0306.2018.01.009
酶法制备大豆肽的相对分子量分布及降压作用研究
Study on relative molecular weight distribution and depressor effect of soybean peptide prepared by enzymatic method
摘要
Abstract
Objective:To study the double enzyme compound enzyme preparation of soybean protein isolated soy peptide segments of the relative molecular weight distribution and activity of antihypertensive effect of experimental hypertensive rats.Methods:Through the optimization of single factor experiment,orthogonal experiment was used to optimize the compound enzyme process,the enzyme solution of angiotensin converting enzyme (ACE) inhibitory rate was as the index to optimize the process by ultrafiltration and nanofiltration,the best molecular fragments left nitro arginine (L-NNA) induced hypertension model rats were given different doses of the active fragment of test.Resuhs:The optimum conditions of double enzyme solution was:the ratio of material to liquid 1∶20 g/mL,enzymolysis temperature of 50 ℃,the ratio of enzyme to substrate 3％,enzymatic hydrolysis under the conditions of pH7.0 by bromelain hydrolysis for 2 h,then adding 4％ trypsin enzyme substrate ratio,control the temperature of 40 ℃ and the enzyme pH was 8.0 under the condition of enzymatic hydrolysis for 4 h,the hydrolysis degree was 35.31％ to ACE soybean peptides.After HPLC enzyme molecular weight distribution of the hydrolysates of soybean protein,polypeptide and protein solution containing the separation of the relative molecular mass was mainly in the range of5000～1.0 × 10s Da in double enzyme solution,the relative molecular mass of the main range of protein and peptide hydrolysates were obtained by ultrafiltration in 500～4000 Da,the best active fragment was 1000～3000 Da,pharmacological experiments showed that,compared with the model control group each group had lower blood pressure,and the soybean peptide dose group had significant difference(p ＜ 0.05),when the soybean peptide in high dose group and captopril group.Conclusion:The relative molecular weight of soybean peptides prepared by double enzyme complex hydrolysis is smaller,and the active fraction has a significant hypotensive effect on hypertensive rats.关键词
大豆分离蛋白/双酶复合酶解/大豆肽/降压Key words
soybean protein isolate/double enzyme complex enzymatic hydrolysis/soybean peptide/decompression分类
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王升光,于帅,孟凡刚,李秉润,宋晓光,刘国飞,王广录,代龙,高鹏..酶法制备大豆肽的相对分子量分布及降压作用研究[J].食品工业科技,2018,39(1):46-51,6.基金项目
国家自然科学基金(81073031) （81073031）
