食品工业科技2018，Vol.39Issue(1)：167-171,5.DOI:10.13386/j.issn1002-0306.2018.01.031
微波辅助提取牡丹籽粕多糖工艺优化及其体外抗氧化活性
Optimization of microwave-assisted extraction technology of polysaccharide from tree peony seed meal and evaluation of antioxidant activity in vitro
摘要
Abstract
To develop polysaccharide by microwave-assisted extraction technique and test its antioxidant activity.The polysaccharide was extracted from tree peony seed meal (PA),and that was determined by 3,5-Dinitrosalicylic acid,to investigate extraction time,extraction power,seed meal granularity and the ratio of material to water.Based on these experiments,finally the orthogonal experiments were to optimize extraction condition.Meanwhile,the ability of polysaccharide to scavenge hydroxyl radical was studied to make a preliminary determination of its biological activity.The results showed the effect order of each factor on yield was as follows:stock ratio ＞ extraction power ＞ seed meal granularity ＞ extraction time and the optimal extraction conditions of polysaccharide were 480 W microwave power for 8 min in combination with stock ratio 1∶25 (m/v)and the size of granularity among 120 items,by which the yield of polysaccharide was up to 9.21％.The crude polysaccharide from tree peony seed meal could scavenge DPPH radical and inhibit lipid peroxidation of polyunsaturated fatty from yolk lipoprotein.Thus,the polysaccharide from tree peony seed meal could be developed as a new type of anti-oxidants.This study has provided a technical basis for further development of tree peony seed meal polysaccharide functions and utilization of tree peony resources.关键词
牡丹/籽粕/多糖/微波法/抗氧化活性Key words
tree peony/seed meal/polysaccharide/microwave/antioxidative activity分类
轻工纺织引用本文复制引用
郭香凤,史田,马雪情,张改娜,史国安..微波辅助提取牡丹籽粕多糖工艺优化及其体外抗氧化活性[J].食品工业科技,2018,39(1):167-171,5.基金项目
国家自然科学基金(31372098) （31372098）
河南省教育厅重点研究项目(15A180036) （15A180036）
洛阳市科技支撑计划(1301051A、1401074A). （1301051A、1401074A）
