食品工业科技2018，Vol.39Issue(1)：235-240,252,7.DOI:10.13386/j.issn1002-0306.2018.01.042
超声辅助提取灰灰菜多糖工艺优化及其体外抗氧化活性
Optimization of ultrasonic assisted extraction technology of polysaccharide from Chenopodium album Linn and its antioxidant activity in vitro
摘要
Abstract
Using the extraction rate of polysaccharide as index,optimization of the ultrasonic-assisted water extraction and ethyl alcohol precipitation of polysaccharides from Chenopodium album L.(CAPs)was studied.Based on single-factor experiments,five independent factors were selected as affecting factors during extraction with response surface method.The antioxidant activities of CAPs were evaluated by DPPH radical scavenging activity (DPPH),ABTS radical scavenging activity (ABTS),hydroxyl radical scavenging(· OH).The results showed that the optimal conditions of the ultrasonic-assisted water extraction and ethyl alcohol precipitation of CAPs were as follows:the liquid-solid ratio was 30∶ 1,ultrasonic time was 20 min,ultrasonic temperature was 80 ℃,ultrasonic power was 460 W,concentration of ethyl alcohol was 100％,under these conditions the yield of CAPs was 5.153％.The CAPs had strong scavenging activity against DPPH·,· OH and ABTS· radical,and the IC5o values were 1.899 mg/mL,1.973 mg/mL and 2.827 mg/mL,respectively.Accordingly,the CAPs had a certain antioxidant activity and has a potential value of being exploited as functional food.关键词
灰灰菜多糖/提取工艺/响应曲面法/抗氧化活性Key words
Chenopodium album L.polysaccharides/extraction process/response surface method/antioxidant activity分类
轻工纺织引用本文复制引用
何念武,秦娇娇,王新军..超声辅助提取灰灰菜多糖工艺优化及其体外抗氧化活性[J].食品工业科技,2018,39(1):235-240,252,7.基金项目
陕西省青年科技新星项目(2014KJXX-79) （2014KJXX-79）
陕西省教育厅专项科研计划项目(16JK1242) （16JK1242）
陕西省生物学优势学科专项资金. （）
