食品工业科技2018，Vol.39Issue(1)：253-256,261,5.DOI:10.13386/j.issn1002-0306.2018.01.045
中国传统豆制品中异黄酮的超高效液相色谱-紫外检测器快速定量法
Rapid quantification of isoflavones in traditional Chinese soybean food by ultra-high performance liquid chromatography coupling to the UV detector
摘要
Abstract
An efficient and rapid quantitative method was developed for the evaluation of six types of isoflavones in soy food,including daidzin,glycitin,genistin,daidzein,glycitein and genistein.An UPLC column,BEH C 18 (2.1 mm × 50 mm,1.7 μm) was employed with acetonitrile and 0.1％ acidic H2O(v/v)as mobile phase.The gradient was from 10％ acetonitrile to 45％ acetonitrile during 10 min at a flow rate of 0.3 mL/min.The detection wavelength was selected as 260 nm and the column was operated at 45 ℃.Six standard isoflavones were separated in 7.5 min and good linear relationships were achieved with correlation coefficients of 0.9998～0.9999.The recovery values in spiked samples were in the range of 96.8％ ～ 102.0％.The method was applied in the detection of isoflavones in traditional Chinese soyfood,including bean curd stick,dried toufu,bean curd shin,braised deep-fled bean curd,soft tofu,firm tofu and lactone bean curd.8.67～25.83 g/kg isofavones was detected in the tested soyfood,and up to 88.0％ ～93.4％ of isoflavones components were daidzin and genistin.These results suggested that isoflavones could be rapidly determined by ultra-high performance liquid chromatography coupled with photo-diode array detector in traditional Chinese soy food.关键词
豆制品/异黄酮/超高效液相色谱-紫外检测器(UPLC-UV)Key words
soybean product/isoflavone/ultra-high pressure liquid chromatography coupling to the UV detector(UPLC-UV)分类
轻工纺织引用本文复制引用
张爽,焦妍津,陶冠军,陈洁..中国传统豆制品中异黄酮的超高效液相色谱-紫外检测器快速定量法[J].食品工业科技,2018,39(1):253-256,261,5.基金项目
江南大学食品科学与技术国家重点实验室自由探索资助课题(SKLF-ZZB-201607) （SKLF-ZZB-201607）
国家重大科学仪器专项(2011YQ170067). （2011YQ170067）
